Activation of cyclic nucleotide-gated (CNG) ion channels involves a conformational change in the channel protein referred to as the allosteric transition. The amino terminal region and the carboxyl terminal cyclic nucleotide-binding domain of CNG channels have been shown to be involved in the allosteric transition, but the sequence of molecular events occurring during the allosteric transition ...

BACKGROUND Sorafenib-everolimus combination therapy may be more effective than sorafenib monotherapy for hepatocellular carcinoma (HCC). To better understand this effect, we comparatively profiled the metabolite composition of HepG2 cells treated with sorafenib, everolimus, and sorafenib-everolimus combination therapy. MATERIAL AND METHODS A 2D HRMAS 1H-NMR metabolomic approach was applied to...

The application of direct-current differential electrolytic potentiometry to the nonaqueous titration of amino acids was investigated. The basic character of amino acids in acetic acid was enhanced to permit their direct titration with perchloric acid. A pair of antimony electrodes was used as an indicating system. The shapes of the titration curves obtained were almost symmetrical with sharp p...

We performed analyses of the phenotypic and genotypic relationships focusing on biosyntheses of amino acids, purine/pyrimidines, and cofactors in three Lactobacillus strains. We found that Lactobacillus fermentum IFO 3956 perhaps synthesized para-aminobenzoate (PABA), an intermediate of folic acid biosynthesis, by an alternative pathway.

drought is an important abiotic stress limiting plant performance. generation of reactive oxygen species (ros) is enhanced under stresses. two greenhouse experiments were conducted to evaluate the effect of phytohormones on the changes of antioxidant enzymes and carotenoids in petals of pot marigold (calendula officinalis l.) under drought stress. results showed that the activities of superoxid...

hexavalent chromium is mobile and hazardous in the environment. electrokinetic remediationof chromium (iv)-contaminated soils is intended either to remove or to reduce cr (vi) to cr (iii). this studyexamines the effectiveness of utilizing edta and acetic acid solutions as alternative electrolytes in theelectrokinetic (ek) process, with coupled nano-scale zero-valent iron (nzvi) as a barrier for...

introduction: ulcerative colitis is a chronic inflammation of the large intestine (colon). in patients with ulcerative colitis, ulcers and inflammation of the inner lining of the colon lead to symptoms such as abdominal pain, diarrhea, and rectal bleeding. previous studies have shown that matricaria recutita l. have a series of physiological effects for example spasmolytic, carminative, antioxi...

background and objectives: acetic acid bacteria (aab) are useful in industrial production of vinegar. the present study aims at isolation and identification of acetic acid bacteria with characterization, optimization, and evaluation of their acetic acid productivity. materials and methods: samples from various fruits were screened for presence of acetic acid bacteria on glucose, yeast extract, ...

Mitogillin and the related fungal ribotoxins are highly specific ribonucleases which inactivate the ribosome enzymatically by cleaving the 23-28 S RNA of the large ribosomal subunit at a single phosphodiester bond. The site of cleavage occurs between G4325 and A4326 (rat ribosome numbering) which are present in one of the most conserved sequences (the alpha-sarcin loop) among the large subunit ...