We studied the analytical and clinical performance of six methods for creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB): three immunoassays (Behring, Hybritech, and International Immunoassay Labs); one immunoinhibition assay (Roche); one immunoinhibition/column method (Du Pont); and one electrophoretic method (Beckman). Between-day precision for all kits was poor at the upper reference limit. A...

Background: After acute myocardial infarction, the structural protein T is released considerably longer than cytosolic creatine kinase (CK), CK MB isoenzyme (CK-MB), or lactate dehydrogenase (LDH) and late troponin T release (> 48 hours after onset of chest pain) appears to be less affected by early coronary reperfusion. Objective: To investigate the precision of a single measurement of circula...

BACKGROUND After acute myocardial infarction, the structural protein T is released considerably longer than cytosolic creatine kinase (CK), CK MB isoenzyme (CK-MB), or lactate dehydrogenase (LDH) and late troponin T release (> 48 hours after onset of chest pain) appears to be less affected by early coronary reperfusion. OBJECTIVE To investigate the precision of a single measurement of circula...

The myocardial effect of snake venoms is considered as one of the most common pathogenesis in many cases of snake envenomation. This study was under taken to investigate the effects of snake (Naja naja oxiana) venom on cardiac function in experimental animals. The blood samples from all the rabbits were collected before venom injection and then 140 µg/kg venom of snake (Naja naja oxiana) was in...

To the Editor: Recently, Paul Collinson and colleagues reported some results and perspectives about the diagnosis of myocardial infarction (MI). Here, we want to show some results in a public hospital of the State of Rio Grande do Sul, Brazil. Cardiac troponin T (cTnT) is considered the ‘gold standard’ in the diagnosis of MI since 2000, because of its high specificity for the heart muscle; howe...

Enzyme kinetics for creatine kinase (CK), CK-MB, aspartate aminotransferase (AST), and lactate dehydrogenase (LD) in serum were followed in 14 patients who had suffered acute myocardial infarction and who were given intracoronary streptokinase shortly (mean 4.9 h, SD 2.6 h) after onset of symptoms. In the 10 patients for whom thrombolysis was successful, CK activity peaked earlier (12.8 vs 21.6...

Reported proportionsofcreatine kinase (CK; EC 2.7.3.2) MB isoenzyme (CK-MB) in skeletal muscles range from 0 to 28% (1-6). Recent observations of increased activities of CKMB in marathon runners have renewed the interest in studying the proportions of CK isoenzymes in skeletal muscle (7-9). Because previous studieswere limited to a few locations and a few types of muscles, we have carried out a...

BACKGROUND The prognosis of patients hospitalized with acute myocardial ischemia is quite variable. We examined the value of serum levels of cardiac troponin T, serum creatine kinase MB (CK-MB) levels, and electrocardiographic abnormalities for risk stratification in patients with acute myocardial ischemia. METHODS We studied 855 patients within 12 hours of the onset of symptoms. Cardiac trop...

OBJECTIVES Nearly one-third of healthcare costs are potentially avoidable and would not compromise medical care if eliminated. Therefore, we sought to evaluate the financial impact of reduction in use of creatinine kinase (CK)-MB and myoglobin tests after removing them from the cardiac enzyme order set at a community hospital. METHODS Grand rounds were held, and an email notification was sent...

We report the results of enzyme determinations in sera from 88 patients, 65 of whom showed inconspicuous reconvalescence, 14 who had myocardial infarction within 24 h (MI 1) after bypass surgery, and nine with myocardial infarction between 24 and 48 h postoperatively (MI 2). We wanted to determine whether the consequent measurement of activities of total creatine kinase (CK), CK isoenzyme MB (C...