The chemotherapeutic drug cis-diamminedichloroplatinum (II) covalently binds to DNA resulting in a variety of adducts and cross-links which are thought to be responsible for the toxicity of the drug. We have used the gel mobility shift assay to detect proteins which bind to DNA treated in vitro with cis-diamminedichloroplatinum (II) and have identified two complexes which bind with increased af...

Petri Nets (PNs) and their variations are a graphical, mathematical language that can be used for the specification, analysis and verification of discrete event systems, including Critical Infrastructures (CIs). Colored PNs are an extension of classical PNs that are suitable for modeling and analyzing complex interconnected CIs. Timed PNs are another extension of PNs that support timing constra...

SNARE-dependent membrane fusion requires the disassembly of cis-SNARE complexes (formed by SNAREs anchored to one membrane) followed by the assembly of trans-SNARE complexes (SNAREs anchored to two apposed membranes). Although SNARE complex disassembly and assembly might be thought to be opposing reactions, the proteins promoting disassembly (Sec17p/Sec18p) and assembly (the HOPS complex) work ...

A bis(phenoxy-amine)ZrMe(2) complex (1) was synthesized and activated with B(C(6)F(5))(3) obtaining an ion pair (2) having the MeB(C(6)F(5))(3)(-) counterion in the second coordination sphere as deduced by NMR studies. Both DFT calculations and NMR investigations suggest that the activation is coupled with a change of the binding modality of the tetradentate ligand from cis(N,N)-trans(O,O) in t...

In this paper we propose an approach which allows to define the Requirement Engineering step of a Cooperatif Information System. We used a notion of software engineering: the viewpoints. CIS is a complex system, it involves the cooperation of many stakeholders in a common purpose and each with their own viewpoint. This is why we used the notion of viewpoints, in order to decompose and partition...

Zero kinetic energy (ZEKE) photoelectron spectroscopy of the hydroquinone-water (HQW) complex was carried out to characterize its S(1)-S(0) resonantly enhanced multiphoton ionization (REMPI) spectrum in terms of the cis and trans conformers. The ZEKE spectra of the hydroquinone isomers show differences in the Franck-Condon (FC) activity of a few ring modes, viz., modes 15, 9b, and 6b, due to th...

Collaborative information seeking (CIS) has become common and occurs when people search the Web together to pursue a complex and exploratory task, such as scientific research, holiday planning, etc. Sensemaking activities are highly involved in these tasks but we have limited understanding of collaborative sensemaking behaviour in the CIS context. In this paper, we describe an exploratory study...

This paper presents AMICI, a new Assessment/analysis platform for Multiple Interdependent Critical Infrastructures (CIs). Its architecture builds on our previous work and uses Emulab to recreate ICT software and hardware components and Simulink to run the physical process models. Our previous framework is extended with software components to provide a set of capabilities that would enable the a...

Having shown rapid trafficking of aminoglycosides to the Golgi complex in cell culture, we focused on the injurious interaction that occurs when gentamicin administration is preceded by renal ischemia. Using Texas red-labeled gentamicin as a tracer, we determined that 15 min of cellular nucleotide depletion did not significantly increase subsequent uptake. However, cells previously depleted of ...