Histone deposition proteins CAF-1 and Asf-1 impact multiple aspects of chromosome formation and are essential in yeast to prevent DNA damage and avoid chromosomal rearrangement. Using fractionated yeast cell extracts, this MQP attempted to identify stimulatory factors which are necessary and sufficient for histone deposition by CAF-1 and/or Asf1. Identification of these cofactors, would provide...

A method is presented to optimize chromosomal data on a cladogram. This procedure simultaneously considers variation at the nucleotide and locus levels including nucleotide substitution, insertion and deletion, locus insertions and deletion, and gene rearrangement. Locus labeling is not a requirement of the procedure and such annotation will result from the dynamic homology analysis of the chro...

Chromosomal instability (CIN) plays a crucial role in tumor development and occurs mainly as the consequence of either missegregation of normal chromosomes (MSG) or structural rearrangement (SR). However, little is known about the respective chromosomal targets of MSG and SR and the way these processes combined within tumors to generate CIN. To address these questions, we karyotyped a consecuti...

All mature B-cell leukaemias and lymphomas have a clonal Ig gene recombination, and half of them have a reciprocal chromosomal translocation involving the 14q32 locus. The 14q32 translocation partners are variable, such as BCL-2, BCL-1 and BCL-6, thus accounting for the difficulty in molecular detection by the current genomic polymerase chain reaction (PCR) method. To identify B-cell clones eff...

Chromosomal abnormality is the most common genetic cause of male infertility, particularly in cases of azoospermia, oligozoospermia, and recurrent spontaneous abortion. Chromosomal rearrangement may interrupt an important gene or exert position effects. The functionality of genes at specific breakpoints, perhaps with a specific role in spermatogenesis, may be altered by such rearrangements. Str...

Leukemic cell chromosomal findings in 27 infants were analyzed. Among the 18 cases of acute nonlymphoblastic leukemia (ANLL), all but two were classified as monocytic or myelomonocytic. The remaining nine cases were acute lymphoblastic leukemia (ALL), seven lacking the common ALL antigen and two having cytoplasmic immunoglobulin (pre-B phenotype). Twenty-five cases (93%) had an abnormal karyoty...

B-cell chronic lymphocytic leukemia is a clinically, genetically and molecularly heterogonous malignancy. During normal B-cell maturation, chromosomal recombination of the immunoglobulin heavy chain V (variable), D (diversity) and J (junctional) gene, and immunoglobulin kappa and lambda light chain V-J gene rearrangement provides the basis for the tremendous immunologic diversity of B cells. Up...