A fully automated and computationally efficient Pearson's correlation change classification (APC3) approach is proposed and shown to have overall comparable performance with both an average accuracy and an average AUC of 0.89 ± 0.08 but is 3.9 to 7 times faster, easier to use and have low outlier susceptibility in contrast to other dimensional reduction and classification combinations using onl...

The use of molecular marker in the dairy sector is gaining large acceptance as a reliable diagnostic approach for food authenticity and traceability. Using a PCR approach, the rbcL marker, a chloroplast-based gene, was selected to amplify plant DNA fragments in raw cow milk samples collected from stock farms or bought on the Italian market. rbcL-specific DNA fragments could be found in total mi...

Motivation Sanger sequencing is still being employed for sequence variant detection by many laboratories, especially in a clinical setting. However, chromatogram interpretation often requires manual inspection and in some cases, considerable expertise. Results We present GLASS, a web-based Sanger sequence trace viewer, editor, aligner and variant caller, built to assist with the assessment of...

With reversed-phase high-performance liquid chromatography we effected rapid and efficient separation of metabolites present in hemodialysate fluid from uremic patients on the artificial kidney. With an aqueous sodium acetate/methanol gradient as mobile phase, more than 50 ultraviolet-absorbing constituents were resolved in a 70-min chromatogram of a 100-mul sample of dialysate. Many of the met...

Two prenylated indole alkaloids were isolated from the ethyl acetate extracts of a marine-derived fungus Penicillium sp. NH-SL and one of them exhibited potent cytotoxic activity against mouse hepa 1c1c7 cells. In order to detect other bioactive analogs, we used liquid chromatogram tandem mass spectrometry (LC-MS/MS) to analyze the mass spectrometric characteristics of the isolated compounds as...

A 15-year-old girl, who had a left-sided Duane's syndrome, was noted to be profoundly deaf with a severe speech defect. The deafness had been detected at the age of 5 years and necessitated her attendance at a special school. A slight facial asymmetry was present and she adopted an abnormal head posture, turning the head to the left side. Some degree of mental retardation was present but no oth...

A novel o-phthalaldehyde-reactive compound was found in the h.p.l.c. chromatogram of Aplysia kurodai extract. This compound was isolated by ion-exchange chromatography and preparative high-voltage paper electrophoresis. It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine. T...

Paecilomyces lilacinus is a nematophagous fungus currently developed as a biological control agent. In order to evaluate potential toxin production, culture extract and concentrated culture supernatant of P. lilacinus strain 251 were tested against Gram-negative and Gram-positive bacteria. High-performance liquid chromatography analysis was carried out to compare the chromatograms of P. lilacin...

An inaccuracy in the computation of the linear mobile phase velocity rendered the whole content of a recently published article, Q.Z. Du, P.D. Wu, Y, Ito, J. Liq. Chromatogr. & Rel. Technol., 23(19), 2929 (2000), erroneous. Since the stationary phase volume was given, the correct mobile phase velocity values were computed and the new values for the Van Deemter plots of three compounds are liste...

The goal of this paper is to demonstrate a new approach to the chromatographic total antioxidant potential (TAP) assay and chromatographic fingerprints of honeys. The analyte is analyzed using reversed-phase high-performance liquid chromatography with electrochemical (amperometric) detection. The TAP measure was the total surface area of all recorded chromatographic peaks (on the amperometric d...