From the absorption spectra and the excitation spectra of chlorophyll a fluorescence measured (4-77 °K) at different wavelengths (685-760 nm) and their comparison with the emission spectra published elsewhere 23, we confirm that both the Pigment Systems (I and II) contain chlorophyll b, chlorophyll a 670 and chlorophyll a 678. But System II is relatively enriched in chlorophyll b and System I i...

Illumination of 3 day old etiolated radish seedlings with continuous white light results in a progressive accumulation of chlorophyll a and b. Both pigments are bound in a different way to the thylakoid chlorophyll-proteins, which appear parallel to the formation of chlorophylls. By applying the SDS-PAGE method to SDS-digested chloroplasts, it was possible to show that the chloroplasts of radis...

The content of individual chlorophyll and carotenoid pigments is determined in three spinach varieties (Lorelei, Springfield, and Ballet) after processing. Raw spinach and spinach that is steam-blanched for 3, 9, or 15 min is stored frozen at -24 degrees C for 6 months. In addition, spinach is air-dried at 75 degrees C, packed in atmospheric air or nitrogen, and stored at ambient temperature fo...

Chlorophyll b is found in photosynthetic prokaryotes and primary and secondary endosymbionts, although their light-harvesting systems are quite different. Chlorophyll b is synthesized from chlorophyll a by chlorophyllide a oxygenase (CAO), which is a Rieske-mononuclear iron oxygenase. Comparison of the amino acid sequences of CAO among photosynthetic organisms elucidated changes in the domain s...

A reverse-phase h.p.l.c. technique was used to estimate the concentration of chlorophyll b in phytoplankton cultures, fecal pellets of Calanuipacificus, and suspended paniculate matter from the Central North Pacific, Oregon coastal waters, and Dabob Bay (a temperate fjord in Puget Sound, WA, USA). The purpose was to assess the distribution of this pigment in the euphotic zone and its effect on ...