From a wild diploid species that is a relative of wheat, Aegilops speltoides, a 301-bp repeat containing 16 copies of a CAA microsatellite was isolated. Southern blot and fluorescence in situ hybridization revealed that approximately 250 bp of the sequence is tandemly arrayed at the centromere regions of A- and B-genome chromosomes of common wheat and rye chromosomes. Although the DNA sequence ...

We recently described successful molecular diagnosis of B. burgdorferi DNA by nested PCR in urine in patient-derived samples only after extraction with DNAzol (1). The aim of this study was to examine whether Borrelia burgdorferi DNA can also be detected in urine by quantitative one-step real-time PCR (Q-PCR). Q-PCR was performed after extraction with DNAzol (Molecular Research Center Inc., Cin...

not impose additional requirements beyond those imposed by state law. For that reason, this proposed action: • is not a ‘‘significant regulatory action’’ subject to review by the Office of Management and Budget under Executive Order 12866 (58 FR 51735, October 4, 1993); • does not impose an information collection burden under the provisions of the Paperwork Reduction Act (44 U.S.C. 3501 et seq....

BACKGROUND Laparoscopic appendicectomy (LA) rapresents a standard but questionable approach in the treatment of acute appendicitis. The scope of this study is to show our experience using different methods in the mesoappendix and appendix stump treatment. MATERIALS AND METHODS A retrospective analysis of all patient with acute appendicitis that underwent to LA was obtained. The results have b...

Background Carbonic anhydrase (CA) enzymes catalyze the interconversion of CO2 and bicarbonate. These enzymes play important roles in cellular metabolism such as CO2 transport, ion transport, and internal pH regulation. Understanding the roles of CAs in the chemolithotropic betaproteobacteria Ralstonia eutropha is important for the development of fermentation processes based on the bacterium’s ...

Coordinated atomic actions CAAs have been used in a semi formal way for the design of the production cell case study This paper presents a formal speci cation and veri cation of the production cell building on this design However this report is not intended to present yet another formalization of the production cell case study but rather as an approach to formalizing a CAA based system design i...

Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (CAA), and cardiovascular risk factors increase dementia risk. Here we present a...

BACKGROUND Codon usage bias has been described for various organisms and is thought to contribute to the regulation of numerous biological processes including viral infections. HIV-1 codon usage has been previously shown to be different from that of other viruses and man. It is evident that the antiretroviral drugs used to restrict HIV-1 replication also select for resistance variants. We wante...

Termites depend nutritionally on their gut microbes, and protistan, bacterial, and archaeal gut communities have been extensively studied. However, limited information is available on viruses in the termite gut. We herein report the complete genome sequence (99,517 bp) of a phage obtained during a genome analysis of "Candidatus Azobacteroides pseudotrichonymphae" phylotype ProJPt-1, which is an...

Aoki, Y., Kawagoe, Y., Fujimori, N., Tanaka, S., and Suzuki, S. 2015. Monitoring of a single point mutation in the PvCesA3 allele conferring resistance to carboxylic acid amide fungicides in Plasmopara viticola populations in Yamanashi prefecture, Japan. Plant Health Progress doi:10.1094/PHP-RS-140041. The use of the carboxylic acid amide (CAA) fungicide mandipropamid to manage grapevine downy ...