The requirements for a successful regeneration of an osteo-chondral defect could effectively be met by using a bi-layered composite scaffold, able to support proliferation and differentiation of mesenchymal stem cells, while providing a biochemical environment promoting the formations of the two distinct tissues. The novel strategy here presented consists of developing a bio-mimetic scaffolds o...

In this study, we designed a chitosan/alginate/hydroxyapatite scaffold as a carrier for recombinant BMP-2 (CAH/B2), and evaluated the release kinetics of BMP-2. We evaluated the effect of the CAH/B2 scaffold on the viability and differentiation of bone marrow mesenchymal stem cells (MSCs) by scanning electron microscopy, MTS, ALP assay, alizarin-red staining and qRT-PCR. Moreover, MSCs were see...

We previously developed a biomaterial scaffold that could effectively provide seed cells to a lesion cavity resulting from traumatic brain injury. However, we subsequently found that few transplanted human umbilical cord mesenchymal stem cells (hUC-MSCs) are able to migrate from the scaffold to the lesion boundary. Stromal derived-cell factor-1α and its receptor chemokine (C-X-C motif) receptor...

BACKGROUND & OBJECTIVES There is a significant bone tissue loss in patients from diseases and traumatic injury. The current autograft transplantation gold standard treatment has drawbacks, namely donor site morbidity and limited supply. The field of tissue engineering has emerged with a goal to provide alternative sources for transplantations to bridge this gap between the need and lack of bone...

Bone scaffolds play an important role in promoting the healing of large bone defects. However, type scaffold material, drug loaded into scaffold, and method preparation have a significant impact on scaffold's properties. In this study, we developed composite comprising sodium alginate (SA), chitosan (CS), hydroxyapatite (HA). The stent carries vascular endothelial growth factor (VEGF), wrapped ...

Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process bone tissue. graft gold standard repair. However, use has a limited amount produced. Tissue engineering latest method in terms regeneration. three main components, first stem cells that have self renewal ability and multineage differentiation, second bioreactor / growth factor, the...

INTRODUCTION: Severe ligament and tendon injuries are frequently treated with autograft reconstruction. However, biological grafts are not ideal replacements. The use of autograft will result in donor site morbidity. Furthermore, the grafts initially undergo necrosis after the implantation. This is the main reason why the biological grafts often do not provide adequate mechanical strength until...

[Introduction] In cartilage tissue enginnering, a number of studies have shown the importance of selecting the appropriate biomaterials as scaffolds for chondrocyte attachment, for supporting its proliferation and for its synthesis of extra-cellular matrix (ECM). Although a variety of biomaterials, including naturally occurring and synthetic, have been introduced as potential scaffolds for cart...

Biomechanical properties of a vascular graft scaffold can be optimized by studying the effect of genipin crosslinker. Collagen-based scaffolds were prepared from a mixture of rat tail collagen, chitosan and bovine aortic elastin and crosslinked with varying concentrations of genipin. Biomechanical testing results showed that the linear modulus of the uncrosslinked samples had the least modulus ...

We report a versatile approach for covalent surface-assembly of proteins onto selected electrode patterns of pre-fabricated devices. Our approach is based on electro-assembly of the aminopolysaccharide chitosan scaffold as a stable thin film onto patterned conductive surfaces of the device, which is followed by covalent assembly of the target protein onto the scaffold surface upon enzymatic act...