Mitomycin C is an alkylating agent used in cancer chemotherapy that shows some specificity towards hypoxic cells. The therapeutic effects of this compound are thought to result from its metabolic activation by enzymes such as NADPH:cytochrome P-450 reducÃ-ase.In a previous report we described a Chinese hamster ovary cell line resistant to mitomycin C, which had a decreased NADPH:cytochrome P-45...

Non-decoupling effects of heavy particles present in beyond-the-standard models are studied for the triple gauge boson vertices γWW and ZWW. We show from a general argument that the non-decoupling effects are described by four independent parameters, in comparison with the three parameters S, T and U in the oblique corrections. These four parameters of the effective triple gauge boson vertices ...

The aim of the study was to investigate the pre- and during-race nutritional intake of cyclists competing in a 210-km 1-day ultraendurance cycle race. Forty-five endurance-trained male cyclists participated in this dietary survey and completed a 3-day dietary record. Mean reported carbohydrate (CHO) intake over the 3 days before the race (5.6 +/- 1.7 g/kg) was below the recommended guidelines o...

Four commercial samples of distillers dried grains with solubles (DDGS) were sieved. All sieved fractions except for the pan fraction, constituting about 90% of original mass, were then winnowed with an air blast seed cleaner. Sieving was effective in producing fractions with varying composition. As the particle size decreased, protein and ash contents increased, and total carbohydrate (CHO) de...

Reduced host cell reactivation (HCR) of a reporter gene containing 8-oxoguanine (8-oxoG) lesions in Cockayne syndrome (CS) fibroblasts has previously been attributed to increased 8-oxoG-mediated inhibition of transcription resulting from a deficiency in repair. This interpretation has been challenged by a report suggesting reduced expression from an 8-oxoG containing reporter gene occurs in all...

Background and novelty Coagulation Factor VIII (FVIII) is an essential cofactor in the blood coagulation cascade. Inability to produce functional FVIII results in haemophilia A which can be treated with recombinant FVIII [1]. Chinese Hamster Ovary (CHO) cells are the most used cell line for producing complex biopharmaceuticals due to its ability to perform complex post-translational modificatio...

We have generated a truncated form of the erythropoietin receptor (EPO-R), the extracytoplasmic ligand-binding domain, that is secreted from a transfected Chinese hamster ovary (CHO) cell line. The truncated receptor is readily purified from CHO conditioned media as a 33-Kd glycosylated protein, which is converted to a 25-Kd species upon treatment with protein N-glycan glycosidase F. Cross-link...

The aim of this study was to investigate the influence of serial mouth rinsing (MR) with nutritional supplements on cognitive performance (i.e., cognitive control and time perception) during a 40-min submaximal exercise. Twenty-four participants completed 4 counterbalanced experimental sessions, during which they performed MR with either placebo (PL), carbohydrate (CHO: 1.6 g/25 mL), guarana co...

Microfiltration is a ubiquitous and often crucial part of many industrial processes, including biopharmaceutical manufacturing. Yet, all existing filtration systems suffer from the issue of membrane clogging, which fundamentally limits the efficiency and reliability of the filtration process. Herein, we report the development of a membrane-less microfiltration system by massively parallelizing ...