The chicken embryo cell line LSCC-H32 was tested for the propagation and titration of several animal viruses of the families Toga-, Reo-, Rhabdo-, Herpeto-, Orthomyxo-, Paramyxo-, and Poxviridae and compared with secondary chicken embryo cells. The LSCC-H32 cells were demonstrated to be as susceptible for most of the tested viruses as were secondary chicken embryo cells. Both produced comparabl...

The total phospholipid content of the supernatant fluid decreases rapidly with increasing relative centrifugal force when amniotic fluid is centrifuged. Possible explanations for this are discussed. The effect of storage on the total phospholipid content of amniotic fluid at different temperatures and the influence of contamination with blood and meconium are also described.

BACKGROUND Pigs are an optimal animal for conducting biomedical research because of their anatomical and physiological resemblance to humans. In contrast to the abundant resources available in the study of mice, few fluorescent protein-harboring porcine models are available for preclinical studies. In this paper, we report the successful generation and characterization of a transgenic DsRed-Mon...

Background & Aims: Various antiviral drugs such as amantadine are used to treat influenza. This drug is categorized in group C and few researches have been conducted about its toxic effects on human fetus. In the current study, the pathologic effects of the drug as well as drug efficacy in reducing influenza virus titer in the developing chicken embryo were assessed. <br ...

The changes in concentration of oestrone, oestradiol (-17alpha and -17beta), oestrone sulphate and the oestradiol sulphates have been measured in allantoic and amniotic fluids and in maternal peripheral plasma throughout gestation. Oestrone sulphate was the major oestrone present in all of the fluids. It was measurable in allantoic fluid before Day 52 and reached a peak concentration of 475 ng/...

Literature concerning procoagulant activity of the amniotic fluid and pathomechanism of amniotic fluid embolism (AFE) was surveyed and a new concept of its pathogenesis, called the integrated concept of AFE, was presented. According to this concept, two components of the amniotic fluid are involved: 1) apoptosis-affected amniotic cells showing a special role in the initiation of disseminated in...

Objectives: To confirm three point of care commercial tests for rupture of membranes consistently and accurately detect amniotic fluids from pregnant women, and they are practical and easy to use. Methods: Samples of fresh amniotic fluid were collected in a syringe taken at time of Csection and run on kits (ActimTMPROM, AmniSure®, and ROM +plus®) from three commercially available point of care ...

background premature rupture of membranes (prom) is one of the most common complications of pregnancy that has a major impact on pregnancy outcomes. a diagnostic tool that is non-invasive, specific and quick is needed to predict prom. the purpose of this study was to evaluate the diagnostic power of the vaginal washing fluid prolactin for the diagnosis of premature rupture of membranes and to d...

This article discusses possible applications of cells derived from human amniotic fluid in regenerative medicine, specifically in stroke therapy. Recent studies have evaluated amniotic fluid as a viable source for mesenchymal stem cells in the expansion of cell-based transplantation. Laboratory data have demonstrated the ability of amniotic fluid stem cells (AFSC) to act as biobridges or subdur...