Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to gi...

The differentiation of troponin (TN) in cardiac and skeletal muscles of chicken embryos was studied by indirect immunofluorescence microscopy. Serial sections of embryos were stained with antibodies specific to TN components (TN-T, -I, and -C) from adult chicken cardiac and skeletal muscles. Cardiac muscle began to be stained with antibodies raised against cardiac TN components in embryos after...

Antibodies raised against chicken gizzard smooth muscle desmin were shown to be specific by immunofluorescence cytochemistry and immunoautoradiography after two-dimensional polyacrylamide gel electrophoresis. Embryonic chick heart cell cultures (permeabilized with Triton X-100) and enucleated adult chicken erythrocyte ghosts (Granger, B. L., E. A. Rapasky, and E. Lazarides, 1982, J. Cell Biol. ...

AIM The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera. MATERIALS AND METHODS A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein ...

BACKGROUND It is important to identify and immunize susceptible healthcare workers to prevent and control hospital infections. Our aim was to evaluate the specific antibodies against the measles, mumps, and rubella viruses and the varicella zoster virus among healthcare workers in a tertiary-care hospital. PATIENTS AND METHODS A total of 284 healthcare workers (89 men and 195 women; mean age,...

Title of Document: PREVALENCE OF SALMONELLA ON LAYING HEN FARMS AND CONTROL OF COLONIZATION IN POULTRY THROUGH EGG YOLK ANTIBODIES. Jose Alejandro N. Almario, Master’s of Science, 2014 Directed By: Assistant Professor, Dr. Debabrata Biswas, Department of Animal and Avian Sciences In the United States, rates of foodborne illness caused by Salmonella have not changed significantly. One study in t...

Normal mouse sera contain naturally occurring antibodies that are cytotoxic in the presence of rabbit complement for NB1, a cell line derived from a neuroblastoma adrenal metastasis of a spontaneous ovarian teratoma. The anti-NB1 antibodies can be specifically removed from normal mouse sera by absorption of the sera with homogenized brain tissue of mouse, rat, guinea pig, chicken, and man and b...

A significant protection provided by maternal antibodies against avian adenovirus serotypes 8 and 11, previously associated with the inclusion body hepatitis and hydropericardium syndrome, was observed in breeder progenies after challenge at 1 and 7 days of age. At two weeks of age, in the presence of low maternal antibodies against avian adenovirus (AAV), chicken anemia virus (CAV) and infecti...

A sensitive and specific method for detection and quantification of methyl phosphonic acid, p-aminophenyl 1,2,2-trimethyl-propyl diester (MATP) as a model substance for organophosphorus compounds is described. Different procedures for coupling the haptenic group for immunization, purification and immobilization allowed the detection of hapten-specific antibodies. The competitive inhibition enzy...

Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the comm...