We report the design and characterization of a disposable light shielded paper-plastic microfluidic device that can detect nanomolar levels of H2O2 using a smartphone camera and a light sealed accessory. Chemiluminescence reaction of H2O2 with bis(2,4,6-trichlorophenyl)oxalate in the presence of rubrene and imidazole is confined to a paper reaction site where the individual reagents are deliver...

The effect of xanthene dyes on the chemiluminescence from the aerobic indole-3-acetic acid (IAA) oxidation, catalyzed by horseradish peroxidase (HRP), was studied. The rate of IAA oxidation and dye destruction were controlled. It was found that the addition of dyes to the IAA/HRP/O2 system resulted in: (i) the appearance of emission in the region of dye fluorescence, (ii) an increase of the tot...

Through the flow injection analysis experiments, we discovered that an unsaturated complex of Cu(II) and polyamines (spermine, spermidine, putrescine) had a strongly catalytic effect on luminol-H(2)O(2) chemiluminescence (CL) reaction, and that the CL intensity is proportional to the concentrations of polyamines. Based on the automatic formation of an unsaturated complex of polyamines and Cu(II...

The detection of chemiluminescence of human polymorphonuclear leukocytes activated by the nonparticulate stimulus phorbol myristate acetate required the presence of suitable substrate, such as protein or luminol, in the reaction medium. This substrate requirement was met by the addition of human serum or various proteins, such as bovine serum albumin, lysozyme, egg albumin, or immunoglobulin, t...