In recent years, many studies have reported potential associations between cytokine gene polymorphisms and the development, course, and outcome of sepsis, often with apparently conflicting results. The objective of this study was to investigate single nucleotide polymorphism (SNP) in the interleukin (IL)-1β -31 T/C, IL-6 -174 G/C, tumor necrosis factor α (TNF-α) -308 G/A, and interferon γ (IFN-...

We have shown that superparamagnetic iron oxide (Fe3O4) nanoparticles with a surface functionalization of dimercaptosuccinic acid (DMSA) are an effective sorbent material for toxic soft metals such as Hg, Ag, Pb, Cd, and Tl, which effectively bind to the DMSA ligands and for As, which binds to the iron oxide lattices. The nanoparticles are highly dispersible and stable in solutions, have a larg...

O. laqueus was first discovered not long ago in 2005 the Ryuku Islands, Japan. Its geographical distribution and molecular identification are therefore still rarely. Nucleotide sequences based on mt-DNA COI for that have been uploaded GenBank until before this study carried out were only six sequences. Since DNA barcoding of has some advantageous characteristics, aimed to analyse genetic differ...

As part of this laboratory’s honeybee genetics and breeding program, DNA markers are needed for uses such as the identification of populations and species and marker-assisted breeding. In particular, DNA markers are needed to discriminate Varroa-mite-resistant Russian honey bees, imported from fareastern Russia, and their offspring (1), from the other honey bees found in the USA. I tried RAPDs,...

Polymerase chain reaction (PCR)based assays have been developed to identify microscopic organisms that cause disease (8) or have an environmental impact (1). In both cases, DNA is extracted from pooled individuals. The pooled sample has typically been created for bacteria by culturing and for disease vectors by taking advantage of their reproduction in the host. PCRbased assays are being develo...

Analysis of cellular 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dGuo) as a biomarker of oxidative DNA damage has been fraught with numerous methodological problems. This is primarily due to artifactual oxidation of dGuo that occurs during DNA isolation and hydrolysis. Therefore, it has become necessary to rely on using the comet assay, which is not necessarily specific for 8-oxo-dGuo. A highly ...

The shutdown of drinking water treatment plants (DWTPs) at the Langat River Basin, Malaysia, which provides to almost one-third population in basin, is very frequent, especially due chemical pollution river. This study explored sources based on eight specific intake points respective DWTPs suggest an integrated river basin management (IRBM). Analysis Al (250.26 ± 189.24 µg/L), As (1.65 0.93 Cd ...