MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. Version 20 of the miRNA registry miRBase contains 307 mature miRNAs and 200 pr...

shoot:root ratio, dry matter partitioning, growth analysis, and water use efficiency of oat (avena sativa l., cv. walker) was investigated under excessive nitrogen (n) as 200 mg n kg-1 (n1p0), excessive phosphorus as 200 mg p kg-1 (n0p1), and combined 100 mg n+100 mg p kg-1 (n2p2), and the control (n0p0) as check in a pot experiment at dryland agriculture institute, west texas a and m universit...

Gravity formulated as a classical gauge theory is based on the Mach principle in terms of curvature scalar R by A. Einstein. The original idea Einstein limits gravity to act spacetime. However, there exist other possible fields such torsion and non-metricity. aim this paper make compatible comparison between three paradigms: via Einstein–Hilbert action, Teleparallel (TEGR) Coincidence (CGR). In...

Abstract Chromosome segment substitution lines (CSSLs) are valuable genetic resources for quantitative trait loci (QTL) mapping of complex agronomic traits especially suitable minor effect QTL. Here, 162 BC 3 F 7 –BC CSSLs derived from crossing two susceptible parent lines, soybean [ Glycine max (L.) Merr.] ‘Suinong14’ (recurrent parent) × wild ( G. soja Siebold & Zucc.) ZYD00006, were used...

Microbiologists are routinely engaged isolation, identification and comparison of isolated bacteria for their novelty. 16S rRNA sequences of Bacillus pumilus were retrieved from NCBI repository and generated QR codes for sequences (FASTA format and full Gene Bank information). 16SrRNA were used to generate quick response (QR) codes of Bacillus pumilus isolated from Lonar Crator Lake (19° 58' N;...

Since 1990, bioinformaticians have been exploring applications of the Chaos Game Representation (CGR) for visualisation, statistical characterisation and comparison of DNA sequences. We focus on the development of a new computational algorithm and description of new software tool that enables CGR visualisation of frequencies of K-mers (oligomers) in a flexible way such that it is possible to vi...

UNLABELLED MEDUSA is a tool for automatic selection and visual assessment of PCR primer pairs, developed to assist large scale gene expression analysis projects. The system allows specification of constraints of the location and distances between the primers in a pair. For instance, primers in coding, non-coding, exon/intron-spanning regions might be selected. Medusa applies these constraints a...

We developed a new approach for the reconstruction of phylogeny trees based on the chaos game representation (CGR) of biological sequences. The chaos game representation (CGR) method generates a picture from a biological sequence, which displays both local and global patterns. The quantitative index of the biological sequence is extracted from the picture. The Kullback-Leibler discrimination in...

We explored DNA structures of genomes by means of a new tool derived from the "chaotic dynamical systems" theory (the so-called chaos game representation [CGR]), which allows the depiction of frequencies of oligonucleotides in the form of images. Using CGR, we observe that subsequences of a genome exhibit the main characteristics of the whole genome, attesting to the validity of the genomic sig...