In Drosophila melanogaster, the wings apart-like (wapl) gene encodes a protein that regulates heterochromatin structure. Here, we characterize a novel human homologue of wapl (termed human WAPL; hWAPL). The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to t...

Eukaryotic telomerase contains a telomerase reverse transcriptase (TERT) and an RNA template component that are essential for telomerase catalytic activity and several other telomerase-associated factors of which only a few appear to be integral enzyme components [1-3]. The first essential telomerase protein identified was S. cerevisiae Est1p, whose deletion leads to ever-shorter telomeres desp...

New, noninvasive methods for the early detection of urothelial carcinomas of the urinary bladder are needed for the diagnosis, follow-up, and screening of patients with bladder cancer. Detection of the enzyme telomerase in urine could offer these new diagnostic possibilities. The standard technique for detecting telomerase activity is the telomeric repeat amplification protocol (TRAP assay). Be...

Double-stranded RNA viruses face two major problems: the endogenous machinery of its host cells does not replicate RNA genomes, and double-stranded RNA provokes strong cellular defence responses such as interferon induction and gene silencing. The solution for the virus is to keep the genomic double-stranded RNA always in a separate container, an icosahedral viral capsid, and to more-or-less au...

telomerase and systems controlling their activity have been of great attention. there are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (htert), the catalytic subunit of telomerase. therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of htert and doubling time of a serie...

to detect hybrid particles. Although the RNA of dependent virus often replicated to a level about 10 per cent of that of supporting virus,' maturation of intact dependent virus within heterologous capsid was much less efficient. This could be due to a better fit between capsomeres and homologous RNA or it could be due to a spatial relationship between capsomere protein synthesis and the viral R...

Causative relations between human papilloma virus infection and cervical intraepithelial neoplasia Stefan Miladinov Kovachev & Victor Dimitrov Slavov To cite this article: Stefan Miladinov Kovachev & Victor Dimitrov Slavov (2016): Causative relations between human papilloma virus infection and cervical intraepithelial neoplasia, Biotechnology & Biotechnological Equipment, DOI: 10.1080/13102818....

Telomerase activation plays critical roles in tumor growth and progression in part through the maintenance of telomere structure. Indeed, the ubiquitous expression of telomerase in human cancers makes telomerase a promising target for cancer therapy. Genetic, pharmacologic, and antisense methods to inhibit telomerase have been described; however, in most cases, cancer cell death was observed on...

Telomerase adds single-stranded telomeric DNA repeats to chromosome ends. Unlike other polymerases involved in genome replication, telomerase synthesizes DNA without use of a DNA template. Instead, the enzyme active site copies a template carried within the integral RNA subunit of the telomerase ribonucleoprotein (RNP) complex. In addition to providing a template, telomerase RNA has non-templat...

AIMS To investigate the pattern of telomerase activity in hydatidiform mole as compared with normal placenta and choriocarcinoma, and to determine the prognostic significance of telomerase activity in hydatidiform mole. METHODS Telomerase activity in 35 cases of hydatidiform mole, 35 normal placentas, one choriocarcinoma sample, and two choriocarcinoma cell lines (JAR, JEG3) was determined us...