A murine hybridoma has been obtained that produces a monoclonal antibody against the human transferrin receptor. In contrast to previously characterized monoclonal antibodies that recognize the transferrin receptor, this antibody, designated 42/6, blocks the binding of transferrin to its receptor and inhibits the growth of the human T leukemic cell line, CCRF-CEM, in vitro. Inhibition of cell g...

BACKGROUND CXCL12 is a pleiotropic chemokine involved in multiple different processes such as immune regulation, inflammatory responses, and cancer development. CXCL12 is also a potent chemokine involved in chemoattraction of T cells to the site of infection or inflammation. Mammalian target of rapamycin (mTOR) is a serine-threonine kinase that modulates different cellular processes, such as me...

Endothelial cell (EC) barrier dysfunction results in increased vascular permeability, a perturbation observed in inflammatory states, tumor angiogenesis, atherosclerosis, and both sepsis and acute lung injury. Therefore, agents that enhance EC barrier integrity have important therapeutic implications. We observed that binding of high-molecular-weight hyaluronan (HMW-HA) to its cognate receptor ...

We have previously demonstrated that within 24 h of exposure of the CEM/A7R cell line to epirubicin (EPI), MDR1 gene expression is induced. The aim of the current study was to investigate the role of cyclosporin A (CyA) and PSC 833, two biochemical modulators of the classical multidrug-resistant phenotype, in this model. CEM/A7R cells were exposed to EPI in the presence or absence of various co...

Glucocorticoid response in target cells closely correlates with glucocorticoid receptor (GR) level. We have compared the localization of GR (by immunocytochemistry) and GR mRNA levels (by in situ hybridization) in steroid-sensitive and steroid-resistant subclones derived from the human CEM lymphoid cell line. In addition, GR was localized in lymphoid cells from patients with various hematologic...

The metabolism of 5-fluorouracil (FUra) was examined in two human lymphocyte cell lines, CCRF-CEM (T-leukemic) and LAZ-007 (Epstein-Barr virus-transformed B), which have widely differing FUra sensitivities. CEM cells have orotate phosphoribosyltransferase but no uridine or thymidine phosphorylase activity. Consistent with this, the FUra growth inhibition and the synthesis of fluorouridine triph...

Abstract Contrast-enhanced mammography (CEM) is gaining rapid traction following the U.S. Food and Drug Administration approval for diagnostic indications. an alternative form of that uses a dual-energy technique image acquisition after intravenous administration iodinated contrast material. The resulting exam includes dual set images, one appears similar to routine 2D mammogram highlights area...

Starting with an established line of human leukemic lympho blasts (CCRF-CEM), we have developed sublines with varying degrees of resistance to vinblastine by growth in the presence of sublethal concentrations of drug. The resistance of these sublines to vinblastine mangesfrom 10.6-fold to more than 2000-fold when compared with the sensitive parent cells. These vinblastine-resistant cells tend t...

background and objective: the management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. in the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line ht29 as an apoptosis resistant cell line, in v...

the cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. this study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder:andmicropowder. human hgf1-pi53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/ml concentrations of the two bioactive...