Influence of pulsed electric field (PEF) simultaneous to pressure treatment on moisture expression from fine-cut cellular raw material has been investigated. Dependencies of specific conductivity σ, liquid yield Y , instantaneous flow rate v and qualitative juice characteristics at different modes of PEF treatment are discussed. Three main consolidation phases were observed in a case of mechani...

objective(s): similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. reprogramming-related transcription factors, also known as yamanaka factors (oct4, sox2, klf4, and c-myc), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. long non-co...

Cellular accumulation of daunorubicin (DNR), N-trifluoroacetyl-adriamycin-14-valerate, and THP-Adriamycin (THP-ADR) in doxorubicin sensitive and resistant murine leukemic P388 cells was studied with laser excited flow cytometry. Appearance of DNR fluorescence in P388/S cells was rapid in contrast to that of P388/R cells. A comparison of P388/S and P388/R cells incubated for 20-30 min showed tha...

This thesis is divided into three parts , united by the theme of the development of selective inhibitors of mammalian cell DNA polymerase delta (pol S). The first part consists of an investigation of the cytotoxic mechanism(s) of certain 2-substituted adenine analogs , selected on the basis of their inhibitory properties towards DNA polymerase alpha (pol a) and mammalian cell DNA synthesis. The...

Introduction Recombinant DNA techniques have revolutionized the field of microbiology. The techniques are quite helpful to analyse and modify various functions of cells. Using recombinant DNA techniques we have succeeded in analysing and modifying the metabolic functions of micro-organisms [ l]. Focusing on the glyoxalase system we have analysed a glycolytic-methylglyoxal cycle and have produce...

The nutrient sensor O-GlcNAc transferase modifies proteins with the O-GlcNAc moiety. In this issue, Capotosti et al. (2011) reveal that O-GlcNAc transferase not only glycosylates the cell-cycle regulator host cell factor 1 but activates it through proteolytic cleavage, providing a surprising link between metabolism and epigenetic regulation of the cell cycle.

introduction: as an inherited neurodegenerative disease, huntington's disease is accompanied with wide neuronal degeneration in neostriatum and neocortex. progress of the disease causes disabling clinical effects on movements, recognition and physiology of the body, and finally results in death. at this stage of knowledge we are, there is no effective therapeutic strategy for diminishing t...

We have used structure-based design techniques to introduce the drug O(2)-[2,4-dinitro-5-(N-methyl-N-4-carboxyphenylamino) phenyl] 1-N,N-dimethylamino)diazen-1-ium-1,2-diolate (PABA/NO), which is efficiently metabolized to potentially cytolytic nitric oxide by the pi isoform of glutathione S-transferase, an enzyme expressed at high levels in many tumors. We have used mouse embryo fibroblasts (M...

Pulsed electric field (PEF) is commonly understood as a fast, non-thermal, and highly effective method for extraction of intracellular compounds. This treatment involves discharge of high voltage electric pulses of a few microseconds into the food product which is placed or passed between two electrodes (Angersbach et al., 2000). Results of experimental investigation of the electric breakage of...