To avoid donor tissue shortages, ex vivo cultured human corneal endothelial cell (HCEC) transplantation is a promising therapeutic resource. Superparamagnetic iron oxide nanoparticle (SPION) cell labeling assists HCEC transplantation by attaching the posterior corneal stroma in ex vivo animal models. However, possible functional changes of the HCECs following SPION labeling remain to be determi...

let z2 = {0, 1} and g = (v ,e) be a graph. a labeling f : v → z2 induces an edge labeling f* : e →z2 defined by f*(uv) = f(u).f (v). for i ε z2 let vf (i) = v(i) = card{v ε v : f(v) = i} and ef (i) = e(i) = {e ε e : f*(e) = i}. a labeling f is said to be vertex-friendly if | v(0) − v(1) |≤ 1. the vertex balance index set is defined by {| ef (0) − ef (1) | : f is vertex-friendly}. in this paper ...

The present studies were initiated to investigate the changes [3H]deoxythymidine labeling index, primer-dependent DNA polymerase labeling index, and S-G2 transition after treatment of T1699 transplantable mouse mammary tumors with Adriamycin (5 mg/kg) and cyclophosphamide (100 mg/kg). Treatment with these agents resulted in intervals of subnormal tumor cell proliferation as indicated by decreas...

The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using 99mTc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized 99mTc-exametazime. Methods: Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized 99mTc-exametazime. The followi...

cell replacement therapy has provided the basis for future clinical applications to treat central nervous system injuries fallowing by car accidents. induced functional neurons are an option for replacing the lost neurons. the ability to monitor changes in membrane potential is a useful tool for studying neuronal function, but there are only limited options available at present. here, investiga...

a better understanding of the mechanism of chromosomal aberration formation could be obtained by using dna repair inhibitors. immortalized normal human (mrc 5 svi) and ataxia telangiectasia ( at 5 biv a ) fibroblastic cell lines were treated with adenosine arabinoside (ara-a) and cytosine arabinoside (ara-c), both potent inhibitors of dna dsb repair, alone or in combination with x-rays at g2 or...

background: stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal mesenchymal stem cells (mscs) have beencarried out. on the other hand, cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. objectives: the aim of this study was toisolate, characterize, an...

Non-invasive methods to track the progress of stem cell therapies are important in the development of future regenerative therapies. Super-paramagnetic iron oxide particles (SPIOs) have previously been applied to track cells using magnetic resonance imaging (MRI) in vivo in non-regenerative animal models. To the best of the author's knowledge, the present study investigated for the first time, ...

We studied the prognostic value of angiogenesis grading and microvessel density estimation in newly diagnosed multiple myeloma. Seventy-five patients with newly diagnosed myeloma, treated on Eastern Cooperative Oncology Protocol E9486 and Intergroup study 0141 (S9321) at the Mayo Clinic, were studied. Bone marrow microvessels were examined using immunohistochemical staining for von Willebrand f...

PURPOSE To investigate whether apoptosis plays a notable role in degeneration of corneal endothelial cells in patients with Fuchs' dystrophy. METHODS Forty-seven corneal buttons from 41 patients with Fuchs' dystrophy were studied. Nucleus labeling, transmission electron microscopy (TEM), and TdT-dUTP terminal nick-end labeling (TUNEL) were used to detect apoptosis. TEM and TUNEL were performe...