Serologic screening for human T-cell leukemia virus type I (HTLV-I) infection was begun in US blood banks with the licensure of enzyme-linked immunosorbent assays (ELISA) in December 1988. We examined the donation histories of the first 60 Western blot (WB)-confirmed HTLV-I/II positive donors to one blood center and found 8 had made 16 previous donations that scored negative on the screening EL...

The genome of eukaryotes exists as chromatin which contains both DNA and proteins. The fundamental unit of chromatin is the nucleosome, which contains 146 base pairs of DNA associated with two each of histones H2A, H2B, H3, and H4. The N-terminal tails of histones are rich in lysine and arginine and are modified post-transcriptionally by acetylation, methylation, and other post-translational mo...

Currently, the accepted method for Q fever serodiagnosis is indirect immunofluorescent antibody assay (IFA) using the whole cell antigen. In this study, we prepared the recombinant antigen of the 27-kDa outer membrane protein (Com1) which has been shown to be recognized by Q fever patient sera. The performance of recombinant Com1 was evaluated in ELISA by IFA confirmed serum samples. Due to the...

Bitis Arientans are associated with the highest number of morbidity and mortality in Nigeria.The most effective treatments against snakebite is administration antivenom.Antibodies that specific for a single epitope an antigen obtain by isolating antibody-secreting lymphocytes. These lymphocytes found within Splenocytes cells which can be used wide variety immunology-based applications. However,...

conclusions real-time pcr and elisa showed high levels of production of 1a/jfh1 chimeric hcv in the huh7.5 cell culture. the constructed virus can be used for future studies, including the development of new hcv drugs and vaccines. background the e2 glycoprotein is an important encoded hepatitis c virus (hcv) protein that contains three different variable regions. objectives the aim of the pres...