Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been...

Sepharose-bound anti-immunoglobulins, which are potent mitogens for normal adult B cells, are not mitogenic for tumor cells freshly isolated from mice carrying the B-cell leukemia BCL1. However, after 4 or more days of in vitro cultivation, BCL1 cells can be stimulated to divide by either anti-mu or anti-delta antibodies. These results suggest that in vitro cultivation of BCL1 cells results in ...

After cultivation of Streptomyces hydrogenans in the presence of 3H-labelled testosterone, radioactive steroids were extracted separately from the cytosolic, ribosomal and cell wall-membrane fraction of the cells and from the culture medium, respectively. The separation of the steroids was performed by one- and two-dimensional thin layer chromatography (TLC). The identification of the main meta...

Chemically defined Schlegel’s medium was modified to improve production of cell growth and other metabolites that are produced by fluorescent pseudomonad R62 strain. The modified medium does not require pH control as pH changes are kept within ± 0.2 units of the initial pH 7.1 during fermentation. The siderophore production was optimized for the fluorescent pseudomonad strain in the modified me...

Viruses control natural microbial communities. Identification of virus-host pairs relies either on their cultivation or on metagenomics and tentative assignment based on genomic signatures. Both approaches have severe drawbacks when aiming to target such pairs within the uncultured majority. Here we present an unambiguous way to assign viruses to hosts that does not rely on any previous informa...

Introduction Typical methods for monitoring cultivation processes are offline analyses like cell counting, measurement of various substrates and products (e. g. glucose or lactate) as well as the online monitoring of several physical process parameters (temperature, pH-value or the concentration of dissolved oxygen). To improve cell cultivations detailed information about important analytes sho...

Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl2 in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 microM EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells ...

Abstract Cyanobacteria are considered to be promising host organisms perform whole-cell biotransformations and for the sustainable production of value-added compounds. However, their commercial applications, scalable photobioreactors that allow high cell density cultivation, stable long-term catalytic performance, product formation necessary. Cyanobacterial biofilms in capillary reactors presen...

The difficulty associated with the cultivation of most microorganisms and the complexity of natural microbial assemblages, such as marine plankton or human microbiome, hinder genome reconstruction of representative taxa using cultivation or metagenomic approaches. Here we used an alternative, single cell sequencing approach to obtain high-quality genome assemblies of two uncultured, numerically...

Puck and his colleagues (1956, 1957) classified tissue-cultured cells into 2 types, epithelioid (Ep) and fibroblast-like (Fb) cells, and reported that their morphological features were constant through many passages so that they could be supposed to be under genetic control. The HeLa strain derived from a human cervical carcinoma (Gey et al., 1952) is a typical cell line belonging to Ep-type of...