Regulation of capsid disassembly is crucial for efficient HIV-1 cDNA synthesis after entry, yet host factors involved in this process remain largely unknown. Here, we employ genetic screening of human T-cells to identify maternal embryonic leucine zipper kinase (MELK) as a host factor required for optimal uncoating of the HIV-1 core to promote viral cDNA synthesis. Depletion of MELK inhibited H...

The Pink Bollworm Pectinophora gossypiella (Saunders) is a devastating pest in Egyptian cotton field. Insecticide control failure may be happen due to its biological features of hide inside bolls and rarely exposed insecticide application. Thus, bulk infested with larvae collected from fields Sharkia Benysuef governorate. Moreover, py, Op sub lethal concentrations laboratory bioassay. Toxicity ...

Background: CD4 T-lymphocyte apoptosis in Human Immunodeficiency Virus infection may occur due to activation of the intrinsic apoptotic pathway or triggered via death receptors. In this study, differential expression genes involved was investigated on circulating T-lymphocytes HIV-infected patients and healthy controls. Methods: The study included 55 HIV who were (1) asymptomatic antiretroviral...

erated (lanes 3 and 12). The same results were obtained for the other six mRNAs investigated using S2-A2, S3A3, S4-A4, S5-A5, S6-A6, and S7-A7 primer pairs (data not shown). Thus, the results presented in this article show that, depending on the reaction conditions (i.e., enzyme concentration, temperature, and buffer composition), RTases from different manufacturers demonstrate different specif...

In vitro synthesis of cDNA is one of the most important techniques in present molecular biology. Faithful synthesis of long cDNA on highly structured RNA templates requires thermostable and processive reverse transcriptases. In a recent attempt to increase the thermostability of the wt Moloney Murine leukemia virus reverse transcriptase (M-MuLV RT), we have employed the compartmentalized riboso...

Abstract Serine protease inhibitors (serpins), a superfamily of inhibitors, are known to be involved in several physiological processes, such as development, metamorphosis, and innate immunity. In our study, full-length serpin cDNA, designated Haserpin1, was isolated from the cotton bollworm Helicoverpa armigera. The cDNA sequence Haserpin1 is 1176 nt long, with an open reading frame encoding 3...

2',5'-branched RNA was recently proposed as a key Ty1 retrotransposition intermediate, for which cleavage by lariat debranching enzyme (Dbr1p) enables reverse transcription to continue synthesizing the complete Ty1 cDNA. Because dbr1 cells can produce substantial Ty1 cDNA despite lacking Dbr1p, the obligatory intermediacy of branched RNA would require that Ty1 reverse transcriptase (RT) can rea...