When mixed ruminal bacteria were incubated with a pancreatic casein hydrolysate and free amino acids of a similar composition, rates of ammonia production were much greater for peptides than for amino acids. The pancreatic digest of casein was then fractionated with 90% isopropyl alcohol. Hydrophobic peptides which dissolved in alcohol contained an abundance of phenolic and aliphatic amino acid...

Yoghurt strain Lactobacillus LBL-4 cultivated for 8-10 h at pH ~6.0 was investigated as a considerable food-grade source of intracellular aminopeptidase. Cell-free extract manifesting >200 AP U/l was obtained from cells harvested from 1 L culture media. Subtilisin-induced hydrolysates of casein, soybean isolate, and Scenedesmus cell protein with degree of hydrolysis 20-22% incubated at 45°C for...

BACKGROUND Hydrolyzed formulas used to feed infants with cow's milk-allergy can be classified as soy based, extensively hydrolyzed (casein, whey and mixed), and amino-acid based. Their unsatisfactory taste is reported by parents and physicians. OBJECTIVE The aim of this study was to ascertain the palatability of these formulas in a double-blind taste test. MATERIALS AND METHODS Fifty health...

The energy, nitrogen and vitamin nutrition of 36 strains of Rhizobium japonicum were investigated. Most of the strains utilized gluconate, glycerol, arabinose, glucose, mannose, mannitol, sorbitol, galactose and maltose. Pyruvate, succinate, ribose, ribitol, xylose, fructose and lactose were of limited use whereas acetate, propionate, lactate, malate, citrate and sucrose were uniformly inadequa...

A scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. Eighty strains, 10 Micropolyspora faeni, 6 Saccharomonospora viridis, 52 Thermoactinomyces candidus, 7 T. vulgaris, 4 T. sacchari, and 1 T. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. In addition to the cultura...

We previously reported that lysine inhibits in vivo homocitrate synthesis in the lysine bradytroph, Penicillium chrysogenum L(1), and that such feedback inhibition could explain the known lysine inhibition of penicillin formation. In the present study, it was found that dialyzed cell-free extracts of mutant L(1) converted [1-(14)C]acetate to homocitrate. This homocitrate synthase activity was e...

In previous reports from this laboratory, a method for the assay of Dand L-methionine in biologic fluids with Leuconostoc mesenteroides P-60 and Lactobacillus fermenti 36 has been described (1, 2). The present communication describes a method for the determination of cysteine plus cystine in plasma. In a report by other workers, using the medium of Dunn et al. (3), the organisms Lactobacillus b...

In this study, we have shown the microbial alkaline protease production by using isolated Bacillus subtilis. Bacillus subtilis which produces an extracellular alkaline protease was isolated from meet surface purchased from local Egyptian markets. Maximum enzyme activity was achieved when the bacterium was grown on corn steep liquor (2.0 %, w/v) instead of soybean meal (2 %) followed by casein h...

1. Measurements of the net synthesis of glucose plus glycogen from various precursors in slices of glycogen-depleted livers from rats at various stages of development indicated an increase in the gluconeogenic capacity after birth with l-lactate, oxaloacetate, a casein hydrolysate, l-serine, l-threonine, l-alanine and glycerol as substrates. 2. The highest rates of incorporation of (14)C-labell...