It has long been recognized that the relatively severe acid hydrolysis necessary to release steroids from their conjugates in urine may result in considerable losses or transformations (3, 4). The isolation of steroid glucuronides (5-7) and of steroid sulfates (8, 9) from human urines has led to the belief that most, if not all, of the urinary steroid conjugates exist as glucuronides or as sulf...

Selenium deficiency in humans has been associated with various diseases, the risks of which can be reduced through dietary supplementation. Selenium accumulating plants may provide a beneficial nutrient for avoiding such illnesses. Thus, leafy vegetables such as Amaranthus hybridus, Amaranthus sp., Cucurbita maxima, Ipomoea batatas, Solanum villosum, Solanum scabrum, and Vigna unguiculata were ...

CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (K maximum ca. 340 nm) to chrome yellow (A maximum ca. 405 nm) concomitant with hydrolysis of the betalactam ring. This compound offers promise as a diagnostic reagent comparable to other chromogens (PADAC and nitrocefin) for the early detection of betalactamase-producing...

A sensitive and specific enzyme immunoassay for measuring angiotensin II (AII) has been developed as a convenient alternative to a radioimmunoassay. An antiserum to AII was prepared using AII conjugated by carbodi-imide to rabbit serum albumin, and coated on to microwell plates. The labelled antigen was prepared from AII and horseradish peroxidase using the periodate method. This enzyme immunoa...

During in vitro incubation of rat adipose tissue in a medium containing crystalline serum albumin, a thiobarbituric acid-reacting chromogen (presumably malonaldehyde) appears in the medium, reflecting peroxidation of polyunsaturated fatty acids in the tissue. Albumin which has been dialyzed, treated with EDTA and dialyzed, or subjected to gel filtration shows a markedly decreased ability to sup...

1. After extraction of teichoic acid from cell walls of Bacillus licheniformis with dilute alkali, the insoluble residue contains the teichuronic acid and mucopeptide components and a small amount of residual phosphorus. 2. A complex of teichuronic acid and a part of the mucopeptide was isolated from the soluble fraction obtained by lysozyme treatment of alkali extracted walls. 3. Small-molecul...

Carica papaya belonging to the Caricaceae family is an effective medicinal herb that is used as a folk medicine for the treatment of various diseases throughout the world including Bangladesh. It can be used for treatment of a numerous diseases like warts, corns, sinuses, eczema, cutaneous tubercles, glandular tumors, blood pressure, dyspepsia, constipation, amenorrhoea, general debility, expel...

Clinistix is generally coiisidered to be specific for detection aiid rough quanti-tation of glucose in urine. The first reaction, being enzymatic. ittay be assumed to be specific. The second reaction-normally, oxidation of the chromogen by the product of enzyme action on glucose-may also be produced directly by hypo-chlorite (1), fumes of nitric acid (2), or other oxidizing agents acting on the...

The Ehrlich reagent has been used extensively for the estimation of acetylated and free hexosamines. The Morgan-Elson (1) method depends upon reaction of this reagent with a chromogen formed from N-acetylhexosamines under mildly alkaline conditions, while the Elson-Morgan (2) method depends upon the formation of chromogens resulting from the interaction of hexosamines and acetylacetone. Under c...

An enzymatic method for quantitative detection of the reduced form of nicotinamide-adenine dinucleotide (NADH) using surface-enhanced Raman scattering was developed. Under the action of NADH oxidase and horseradish peroxidase, NADH can generate hydrogen peroxide (H₂O₂) in a 1:1 molar ratio, and the H₂O₂ can oxidize a chromogen into pigment with a 1:1 molar ratio. Therefore, the concentration of...