Extracellular vesicles (EVs) mediate intercellular communication through transfer of RNA and protein between cells. Thus, understanding how cargo molecules are loaded and delivered by EVs is of central importance for elucidating the biological roles of EVs and developing EV-based therapeutics. While some motifs modulating the loading of biomolecular cargo into EVs have been elucidated, the gene...

Retromer is an evolutionarily conserved protein complex composed of the VPS26, VPS29, and VPS35 proteins that selects and packages cargo proteins into transport carriers that export cargo from the endosome. The mechanisms by which retromer is recruited to the endosome and captures cargo are unknown. We show that membrane recruitment of retromer is mediated by bivalent recognition of an effector...

Clathrin-coated pits (CCPs) are generally considered a uniform population of endocytic machines containing mixed constitutive and regulated membrane cargo. Contrary to this view, we show that regulated endocytosis of G protein-coupled receptors (GPCRs) occurs preferentially through a subset of CCPs. Significantly, GPCR-containing CCPs are also functionally distinct, as their surface residence t...

The terrorist use of a cargo container to smuggle a nuclear weapon or radiological material which could be used in a radiological dispersion device (RDD) is a serious threat currently being addressed by the US and other governments. The US government has negotiated through the Container Security Initiative (CSI) placing Customs and Border Protection inspectors in 20 major overseas ports to help...

We study a generic one-dimensional model for an intracellular cargo driven by N motor proteins against an external applied force. The model includes motor-cargo and motormotor interactions. The cargo motion is described by an over-damped Langevin equation, while motor dynamics is specified by hopping rates which follow a local detailed balance condition with respect to change in energy per hopp...

Penerapan protokol kesehatan di segala aktivitas merupakan syarat mutlak saat menghadapi kondisi baru masa pandemi Covid-19. Kajian ini bertujuan untuk memberikan rekomendasi penanganan pelayanan penumpang dan barang pelabuhan selama dalam rangka menekan penularan Metode yang digunakan analisis deskriptif menggunakan survey online. Variabel diamati adalah kapal secara umum, terminal penumpang, ...

BACKGROUND Endocytosis allows the import and distribution of cargo into a series of endosomes with distinct morphological and biochemical characteristics. Our current understanding of endocytic cargo trafficking is based on the kinetics of net cargo transport between endosomal compartments without considering individual endosomes. However, endosomes form a dynamic network of membranes undergoin...

The directional response and roll stability characteristics of a partly filled tractor semi-trailer vehicle, with cylindrical tank, are investigated in various maneuvers. The dynamic interaction of liquid cargo with the tractor semi-trailer vehicle is also evaluated by integrating a dynamic slosh model of the partly filled tank with five-degrees-of-freedom of a tractor semi-trailer tank mode...

In this study a hierarchical hub location problem with two layers is considered. The first layer includes small hubs and the second one includes a star-shaped network central hub. The considered case is a cargo delivery network where there is hierarchy between hubs. All the hubs and links are capacitated and there are three kinds of commodities for each of which there is a special kind of vehic...

Intracellular organelle transport is driven by motors that act upon microtubules or microfilaments. The microtubulebased motors, cytoplasmic dynein and kinesin, are believed to be responsible for retrograde and anterograde transport of intracellular cargo along microtubules. Many vesicles display bidirectional movement; however, the mechanism regulating directionality is unresolved. Directional...