Objective(s) Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored. Materials and Methods Adherent cells were isolated from the collagenase digests of adipose tiss...

purpose: to separate and characterize orbital fat-derived stem cells (ofscs) and compare its cellular and molecular properties with those of bone marrow-derived mesenchymal stem cells (mscs) and adipose-derived stem cells (ascs). methods: stem cells from orbital fat, abdominal fat and bone marrow aspirates have been isolated. we assessed stem cell specific cell surface markers using cytometry, ...

background: as condition and component of culture determine fate map of spermatogonial stem cells (sscs), the aim of this study was to evaluate of growth factors gdnf, lif and ra on proliferation and differentiation of ssc. materials and methods: sscs were cultured in two groups: the first group gdnf and lif and the second group ra. the number of clumps and colony formation was monitored during...

objective: evaluating the effects of p-benzoquinone and hydroquinone on the runx2 expression and osteoblastic differentiation of human marrow derived mesenchymal stem cells (mscs). materials and methods: bone marrow mscs obtained by cultivating marrow mononuclear cells, were exposed to 10μm of either p-benzoquinone or hydroquinone. following chemical treatment, runx2 gene expression was assess...

background: stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal mesenchymal stem cells (mscs) have beencarried out. on the other hand, cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. objectives: the aim of this study was toisolate, characterize, an...

background: the anticancer agent imatinib (im) is a small molecular analog ofatp that inhibits tyrosine kinase activity of platelet derived growth factors (pdgfs)and stem cell factor (scf) receptor in cancer cells. however these factors have a keyrole in regulating growth and development of normal sertoli, leydig and germcells.objective: the aim of this study was to determine cell viability, pd...