To better understand the size differentiation mechanism of adult male prawn Macrobrachium nipponense, transcriptome sequencing was performed from second pereiopod, hepatopancreas, and muscle M. nipponense with significant differences in (large: 1.47 ± 0.12 g; small: 0.28 0.04 g). A total 46,557 unigenes an average length 1,312 bp were obtained. Based on pereiopod large (LP) versus small (SP), (...

Traditional Chinese medicine (TCM) had demonstrated effectiveness in the prevention and control of COVID-19. Statistics showed that Ephedra Glycyrrhiza were frequently used treatment We hypothesized Ephedra-Glycyrrhiza drug pair is a potential choice for Here, 112 active compounds identified from via network pharmacology approach. enrichment analysis these might participate cAMP, PI3K-Akt, JAK-...

D(1) dopamine receptors are primary mediators of dopaminergic signaling in the CNS. These receptors internalize rapidly following agonist-induced activation, but the functional significance of this process is unknown. We investigated D(1) receptor endocytosis and signaling in HEK293 cells and cultured striatal neurons using real-time fluorescence imaging and cAMP biosensor technology. Agonist-i...

The Galpha protein Gpa1 governs the cAMP-PKA signaling pathway and plays a central role in virulence and differentiation in the human fungal pathogen Cryptococcus neoformans, but the signals and receptors that trigger this pathway were unknown. We identified seven putative proteins that share identity with known G protein-coupled receptors (GPCRs). One protein, Gpr4, shares limited sequence ide...

Abstract Rice false smut caused by Ustilaginoidea virens is one of the widespread rice diseases across globe in recent years, however, we know little about its molecular mechanism infection. The cAMP signaling pathway functions directly development and formation infectious structures to regulate infection process many pathogenic fungi. In order investigate role U. , UvCap1, a cyclase-associated...

Most neurons possess a single, nonmotile cilium that projects out from the cell surface. These microtubule-based organelles are important in brain development and neurogenesis; however, their function in mature neurons is unknown. Cilia express a complement of proteins distinct from other neuronal compartments, one of which is the somatostatin receptor subtype SST(3). We show here that SST(3) i...

Learning-induced synaptic plasticity commonly involves the interaction between cAMP and p42/44MAPK. To investigate the role of Rap1 as a potential signaling molecule coupling cAMP and p42/44MAPK, we expressed an interfering Rap1 mutant (iRap1) in the mouse forebrain. This expression selectively decreased basal phosphorylation of a membrane-associated pool of p42/44MAPK, impaired cAMP-dependent ...

Ciliary neurotrophic factor and bone morphogenetic proteins induce astrocytogenesis in the developing rat brain by stimulating STAT- and Smad-dependent signaling, respectively. We previously found that stimulation of the cAMP-dependent signaling pathway also triggers differentiation of cerebral cortical precursor cells into astrocytes, providing an additional mechanism to promote astrocyte diff...

objective: different signaling pathways have been identified that are involved in the cellular response to opiates. the mitogen-activated protein kinase pathway is one of the most important signaling pathways underlying the neuronal response to opiates. micrornas (mirnas) are considered to be post-transcriptional regulators of gene expression with paramount significance, which plays key roles i...

adrenoceptor ( AR) signaling is finely regulated to mediate the sympathetic nervous system control of cardiovascular function. In neonatal cardiac myocytes, 1AR activates the conventional Gs cAMP pathway, whereas 2AR sequentially activates both the Gs and Gi pathways to regulate the myocyte contraction rate. Here, we show that phosphodiesterase 4D (PDE4D) selectively impacts signaling by 2AR in...