Comparison of natural plant and callus extracts of Solanum trilobatum L. was studied against two bacteria and fungi, for their antimicrobial activity using cup diffusion method. Various solvents such as chloroform, petroleum ether and ethanol were used. The leaf and stem segments of the plant were culturedon Murashige and S koog basal medium supplemented with various growth regulators. Maximum ...

The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, ...

Feasible and efficient tissue culture plays an important role in plant genetic engineering. Wheat (Triticum aestivum L.) immature embryos (IMEs) are preferred for tissue culture to mature embryos (MEs) because IMEs easily generate embryogenic callus, producing large number of plants. The molecular mechanisms of regulation and the biological pathways involved in embryogenic callus formation in w...

BACKGROUND Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model. METHODS Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tib...

Ethanolic extract of root and root derived callus extracts of Premna serratifolia L were investigated against paracetamol induced oxidative stress and hepatotoxicity in blood and liver of male albino rats. The root explants were cultured on MS medium supplemented with various combination and concentration of plant growth regulators. The best callus biomass was observed from the MS medium supple...

This study addresses the hypothesis that callus formation, patterning, and mineralisation are impaired during the early phase of critical sized bone defect healing, and may relate to inter-fragmentary tissue strains within the bone defect area. Twenty four 12 week old Sprague Dawley rats were used for this study. They were divided into two groups defined by the femur bone defect size: (i) 1 mm ...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

Fracture healing requires two major pre-requisites; sufficient blood supply and mechanical stability [1-2]. Optimum mechanical conditions for fracture healing remain unknown, but micro-movements of the fracture fragments are significant to a developing healing callus [2-3]. This study uses Finite Element analysis to evaluate an idealized neck fracture callus, for single, and two lag screw intra...

In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at l...