Mark D. Gibson, Tony J. Ward, Amanda J. Wheeler, Judy R. Guernsey, Matt P. Seaboyer, Patrick Bazinet, Gavin H. King, Neil K. Brewster, James Kuchta, Ryan Potter, Dave M. Stieb Atlantic RURAL Centre, Community Health and Epidemiology, 5790 University Avenue, Dalhousie University, Halifax, Nova Scotia, Canada, B3H 1V7. Center for Environmental Health Sciences, 32 Campus Drive Missoula, University...

Kanishka Bhaduri, Kamalika Das, Kirk Borne, Chris Giannella, Tushar Mahule, Hillol Kargupta Mission Critical Technologies Inc., NASA Ames Research Center, MS 269-1, Moffett Field, CA-94035 Email:[email protected] Stinger Ghaffarian Technologies Inc., NASA Ames Research Center, MS 269-3, Moffett Field, CA-94035 Email:[email protected] Computational and Data Sciences Dept., GMU, VA-...

This study characterized the cytosolic free Ca2+ concentration ([Ca2+]i) in NaCN-treated human A-431 cells. The resting [Ca2+]i was 85 +/- 8 nM (n = 141) in untreated cells at 37 degrees C, determined with the fura-2 fluorescence probe. When cells were treated with NaCN, [Ca2+]i increased in a time- and NaCN concentration-dependent manner. When cells were exposed to 10 mM NaCN for 10 min, [Ca2+...

Nutrient-stimulated insulin secretion is dependent upon the generation of metabolic coupling factors in the mitochondria of the pancreatic B cell. To investigate the role of Ca2+ in mitochondrial function, insulin secretion from INS-1 cells stably expressing the Ca2+-sensitive photoprotein aequorin in the appropriate compartments was correlated with changes in cytosolic calcium ([Ca2+]c) and mi...

Intercellular Ca2+ signaling in intact salivary glands of the blowfly Calliphora erythrocephala was studied by fluorimetric digital imaging combined with microinjection of putative messenger molecules. Iontophoretic injection of D-myo-inositol 1,4, 5-trisphosphate (InsP3) into salivary gland cells evoked regenerative intercellular Ca2+ waves that spread through the impaled cell and several rows...

In airway epithelial cells, extracellular ATP (ATPo) stimulates an initial transient increase in intracellular Ca2+ concentration that is followed by periodic increases in intracellular Ca2+ concentration (Ca2+ oscillations). The characteristics and mechanism of these ATP-induced Ca2+ responses were studied in primary cultures of rabbit tracheal cells with digital video fluorescence microscopy ...

C62B rat glioma cells respond to muscarinic cholinergic stimulation with transient inositol phosphate formation and phospholipase A2-dependent arachidonic acid liberation. Since phospholipase A2 is a Ca2+-sensitive enzyme, we have examined the role of the agonist-stimulated Ca2+ response in production of the arachidonate signal. The fluorescent indicator fura-2 was used to monitor changes in cy...

The precise mechanisms by which nitric oxide (NO) decreases free [Ca2+]i, inhibits Ca2+ influx, and relaxes vascular smooth muscle are poorly understood. In rabbit and mouse aorta, agonist-induced contractions and increases in [Ca2+]i were resistant to nifedipine, suggesting Ca2+ entry through non-L-type Ca2+ channels. Relaxations to NO were inhibited by thapsigargin (TG) or cyclopiazonic acid ...