Our purpose was to determine whether the action of oxidative free radicals released by endothelial cells and vascular smooth muscle cells grown in culture could be responsible for certain modifications to low density lipoprotein (LDL). In these experiments we showed that after a 48-hour incubation with human umbilical vein endothelial cells or bovine aortic smooth muscle cells, human LDL: 1) be...

Commiphora molmol oleoresin (Family Burseraceae) is traditionally used for treatment of many ailments such as ulcers, abscesses and wounds. The aim of this study was to evaluate the antimicrobial and antioxidant activities of C. molmol extracts in vitro (essential oils and ethanol extract). The antimicrobial activities of extracts were evaluated by disc diffusion and micro-broth dilution assays...

The present study was aimed to see the effect of butylated hydroxytoluene (BHT) as semen additives during cryopreservation of Hariana Bull semen. Twenty four ejaculates from two Hariana bulls extended with glycerolated egg yolk tris (GEYT). Extended samples were split into three aliquots. One aliquot was kept as control while other two aliquots were supplemented with 0.5 mM (T1) and 1.0 mM (T2)...

The forkhead box (Fox) proteins are a growing family of transcription factors that have important roles in cellular proliferation and differentiation and in organ morphogenesis. The Fox family members hepatocyte nuclear factor (HNF)-3beta (Foxa2) and HNF-3/forkhead homolog (HFH)-8 (FREAC-1, Foxf1) are expressed in adult pulmonary epithelial and mesenchymal cells, respectively, but these cells d...

Long-term feeding of butylated hydroxytoluene (BHT) to rats and mice has been linked to the enhancement of the incidence of liver tumors. It is shown in this paper that in the liver, urinary bladder and thyroid of the male Wistar rat, feeding the highest tolerated doses of BHT for 30 days does not lead to detectable increases in [3H]thymidine labeling. On the other hand, treatment of rats with ...

This study was conducted to evaluate the oxidative stability of virgin coconut oil (VCO) during oven test. VCO with and without antioxidants were subjected to oven test at 63°C over a 40 day-storage. The chemical parameters namely peroxide value (PV) and specific absorptivity of conjugated dienes (CDs) and conjugated trienes (CTs) were used to asses VCO stability. VCO samples treated with the m...

Lipoxygenase possessing dual catalytic activities, i.e. dioxygenase and hydroperoxidase, was purified from the cytosols of term placentas from non-smoking women. Concanavalin A affinity chromatography followed by phenyl-Sepharose CL-4B chromatography resulted in the separation of one hydrophobic and one non-hydrophobic isoenzyme. The concanavalin A-purified enzyme was used in all subsequent exp...