A subunit vaccine candidate was produced from Brucella suis 145 (biovar 4; expressing both the A antigen of Brucella abortus and the M antigen of Brucella melitensis). The preparation consisted mostly of polysaccharide (PS; >90% [wt/wt]; both cell-associated PS and exo-PS were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. Vaccinated mice were protected (these...

In Turkey, where brucellosis is endemic, a comparison of conventional and molecular genotyping methods has not been published to date. In this study, we investigated the efficacy of single nucleotide polymorphism (SNP) analysis of rpoB gene in the genotyping of Brucella melitensis strains by sequencing. In light of the molecular genotyping method available now in Turkey, the adequacy of serolog...

Although pulmonary symptoms accompany up to 16% of cases of infection with Brucella melitensis, pleural effusion has rarely been reported. A 12 year old girl had brucellosis with pulmonary disease and a pleural effusion. The pleural fluid was clear and straw coloured with 2700 leucocytes/mm3 (93% lymphocytes), a protein level of 48 g/1, and a glucose concentration of 4.1 mmol/l. Culture of the ...

objective: the brucella melitensis virb operon, encoding a type iv secretion system (t4ss), is required for intracellular replication and persistent infection in the mouse model. the product of the second gene of the virb operon, virb2, is predicted to be localized at the bacterial surface, where they could potentially interact with host cells. studies to date have focused on characterization o...

background: dna vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. bacteria of the genus brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. methods: to evaluate the use of a dna immunization strategy for protection against brucellosis, a plasmi...

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS prot...

Many pathogenic bacteria use a regulatory process termed quorum sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection of, and response to, these molecules depends on transcriptional regulators belonging to the LuxR family. Such a system has been discovered in the intracellular pathogen Brucella ...

The response regulator OtpR is critical for the growth, morphology and virulence of Brucella melitensis. Compared to its wild type strain 16 M, B. melitensis 16 MΔotpR mutant has decreased tolerance to acid stress. To analyze the genes regulated by OtpR under acid stress, we performed RNA-seq whole transcriptome analysis of 16 MΔotpR and 16 M. In total, 501 differentially expressed genes were i...

Brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. A dichloromethane extract of spent culture supernatant from B. melitensis induces bioluminescence in an Escherichia coli acyl-homoserine lactone (acyl-H...

The participation of an endotoxin in the pathogenesis of brucellosis has received scant recognition although clinical observations have suggested that a Brucella toxin might be responsible for some of the manifestations in the acute phases of the disease. This has been especially obvious in patients whose illness has been due to Brucella melitensis. In general, brucellosis caused by Br. meliten...