Bovine serum albumin (BSA) and fatty acid-free human serum albumin (HSAFAF) reduce the K(m) values for UGT2B7 substrates by sequestering inhibitory long-chain fatty acids released by incubations of human liver microsomes (HLM) and HEK293 cells expressing this enzyme. However, the scope of the "albumin effect" is unknown. In this investigation we characterized the effects of albumin on the kinet...

The novel pyridine bearing schiff base substituted metal-free (9), zinc(II) phthalocyanine (10), and its quaternized derivative (11) were designed synthesized. These phthalocyanines fully characterized by spectroscopic methods (FT-IR, UV-Vis, MALDI-TOF, 1H NMR). photo-physicochemical properties of these investigated in both DMSO DMF for 10 aqueous solution 11. addition Schiff groups as peripher...

Lifetime prolongation for hydrophobic drug carriers has been the focus of interest for many years. Poloxamer (Pluronic F68, PF68) has been employed in this study for modifying the surface of magnetic poly(d,l-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with human serum albumin (HSA) model drug. Surface characteristics of untreated and PF68 treated NPs were analyzed by size, zeta ...

Interaction of cellular membranes with biointerfaces is vital importance for a number medical devices and implants. Adhesiveness these surfaces cells often regulated by depositing layer bovine serum albumin (BSA) or other protein coatings. However, anomalously large separations between phospholipid the in various conditions buffers have been observed, which could not be understood using availab...

The interactions of human and bovine serum albumins (HSA and BSA) with various drugs have received great attention now a days due to their significant effect in the biomedical field. Detailed information about the interactions of drugs with serum albumin can be deducted from the data of fluorescence quenching studies, UV-Vis, and circular dichroism spectroscopy. Among the all fluorescence spect...

The authors measured ionic current blockages caused by protein translocation through voltage-biased silicon nitride nanopores in ionic solution. By calculating the mean amplitude, time duration, and the integral of current blockages, they estimated the relative charge and size of protein molecules at a single molecule level. The authors measured the change in protein charge of bovine serum albu...

Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgG(I125) and RAM-nanodiamond-BSA(I125) complexes are stable in blood serum and the immobilized proteins retain their biological activity. It was shown that the RAM-nanodiamond-BSA(I125) complex is able to bi...

Protein-based particles are one of the most important research topics in nanomedicine, being used especially as drug delivery systems. From wide variety proteins, albumins offer several advantages biomedical applications due to their special properties. Albumin nanoparticles play an role carriers chemical and biomolecular drugs, such anticancer drugs; many advantages, biocompatibility biodegrad...

A method utilizing bovine serum albumin (BSA) as buffer additive for chiral separation by means of capillary electrophoresis is described. Parameters that affect chiral separation, such as buffer pH, buffer concentration, BSA concentration, and organic modifier, are investigated. Baseline resolution of ephedrine-pseudoephedrine and norephedrine-norpseudoephedrine isomers are achieved in an unco...

Serum albumin is one of the most widely studied proteins. It is the most abundant protein in plasma with a typical concentration of 5 g/100 mL and the principal transporter of fatty acids in plasma. While the crystal structures of human serum albumin (HSA) free and in complex with fatty acids, hemin, and local anesthetics have been characterized, no crystallographic models are available on bovi...