In previous work the existence of free thyroxine in human serum was verified by dialysis through cellophane; the concentration of the unbound hormone in normal human serum was found to approximate one-thousandth of the total thyroxine (1). This direct measurement of free thyroxine was in reasonable agreement with the theoretical value computed from data on the per cent of total thyroxine bound ...

The reaction of tribromoarsenazo(TB-ASA) with serum albumin in the presence of emulgent OP was studied by spectrophotometry. In a Britton-Robinson buffer solution at pH 2.9, tribromoarsenazo and bovine serum albumin can immediately form a red compound in the presence of emulgent OP with a maximum absorption wavelength at 354 nm. The presence of emulgent OP can increase the reaction sensitivity ...

The rapid development of surface sensitive biosensor technologies requires optimum control of surface modification to provide reliable and reproducible results. With the aim to assemble a quartz crystal microbalance (QCM)-based protein biosensor, we focus our attention on sulfide receptor and its integration with the surface of the electrode. Here, we present different surface modification proc...

The interaction of the immobilized triazine dye Cibacron Blue 3G-A with rat, rabbit, sheep, goat, bovine and human serum albumins was studied by affinity gel electrophoresis. Dissociation constants were estimated in each instance and showed human serum albumin to have a significantly higher affinity for the dye than did albumin from any other species. Pretreatment of the defatted proteins with ...

The albumin molecule, in contrast to many other plasma proteins, is not covered with a carbohydrate moiety and can bind and transport various molecules of endogenous and exogenous origin. The enzymatic activity of albumin, the existence of which many scientists perceive skeptically, is much less studied. In toxicology, understanding the mechanistic interactions of organophosphates with albumin ...

The binding of several anticandidal peptides containing N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) to serum proteins was studied using equilibrium dialysis. The affinity of these FMDP-peptides for serum albumin was low and well correlated with their biological activity against Candica albicans ATCC 26278 in serum albumin solution. This binding did not affect the biological activi...

Protein aggregates are a major risk factor for immunogenicity. Until now most studies on aggregate-driven immunogenicity have focused on linking physicochemical features of the aggregates to the formation of anti-drug antibodies. Lacking is however, basic knowledge on the effect of aggregation on the biodistribution and clearance of therapeutic proteins in vivo. The aim of current study was to ...

The competitive adsorption of collagen and bovine serum albumin (BSA) on surfaces with varied wettability was investigated with imaging ellipsometry, and ellipsometry. Silane modified silicon surfaces were used as substrates. The results showed that surface wettability had an important effect on protein competitive adsorption. With the decrease of surface wettability, the adsorption of collagen...

Serum albumin exists in the native or N conformation between pH 5 and 7. As the pH is lowered from 5 to 3.5, the protein undergoes a conformational change resulting in expansion, known as the N = to F (partially acid expanded) transition. As the pH is lowered still further to 2, the protein continues to expand. In the present study, using the techniques of circular dichroism, fluorescence, and ...