In mucosal immunology, the importance of homing of specific T and B lymphocyte memory cells back to their antigen’s tissue of origin has long been established (1). Elucidation of the mechanisms of such homing and their role in pathogenesis and immunodeficiency may provide targets for immunotherapy or vaccines (2). Recently, there has been intense interest in lymphocyte homing during the pathoge...

There is currently no clear in vitro model to study the Th2 status atopic dermatitis (AD). Staphylococcus aureus, skin-homing cutaneous lymphocyte-associated antigen (CLA)+ memory T cells and IL-13 are key players AD. Circulating CLA+ reflect abnormalities We have studied CLA+/- T-cell response staphylococcal enterotoxin B (SEB) when cocultured with autologous lesional epidermal from adult non-...

Monoclonal antibodies specific for the retinal S-antigen were obtained by hybridization of spleen cells from a BALB/c mouse immunized with bovine S-antigen and NS-1 myeloma cells. Five selected antibodies specifically labeled the photoreceptor cells of the retina by immunofluorescence. Whereas antibody S9E2 only reacted with bovine S-antigen, the other antibodies showed interspecies cross-react...

The reactions of human and bovine retinal S-antigen (S-ag) with polyclonal rabbit antibodies were compared in ELISA, immunoblotting and inhibition ELISA. Titrations in ELISA with plastic-adsorbed S-ags revealed that the great majority (70-100%) of human S-ag epitopes and anti-human S-ag antibodies were cross-reactive. In contrast, the cross-reactivity of bovine S-ag (25%) and anti-bovine S-ag a...

Lymphocyte development occurs in a stepwise progression through distinct developmental stages. This ordered maturation ensures that cells express a single, non-autoreactive antigen receptor, which is the cornerstone of a diverse adaptive immune response. Expression of a mature antigen receptor requires assembly of the antigen receptor genes by the process of V(D)J recombination, a reaction that...