Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a fastidious bacterium that replicates slowly and requires special conditions to grow in the laboratory. Borrelia isolation from clinical material is a golden standard for microbiological diagnosis of borrelial infection. Important factors that affect in vitro borrelia growth are temperature of incubation and number o...

In this study, two halophilic bacterial strains isolated from saline habitats in Argentina grew in the presence of gas oil. They were identified as Halomonas spp. and Nesterenkonia sp. by 16S ribosomal RNA sequencing. Chemotaxis towards gas oil was observed in Halomonas spp. by using swimming assays.

Determination of the infection rate ixodid ticks with tick-borne borreliosis pathogens and determination belonging to pathogenic genotype by PCR is an important component for monitoring, risk assessment control epizootic situation Lyme in different territories. The results testing optimization internal laboratory protocol classical polymerase chain reaction identification disease are presented....

Fifty-nine Borrelia burgdorferi sensu lato culture isolates collected from northeastern China were characterized by 5S-23S rRNA intergenic spacer restriction fragment length polymorphism (RFLP) analysis and reactivity with monoclonal antibodies (MAbs). Among 59 culture isolates, 30 (50.8%) were Borrelia garinii and 17 (28.8%) were Borrelia afzelii, 2 were mixtures composed of B. garinii with RF...

tick-borne diseases like tick-borne relapsing fever are a public health problem in iran. its occurrence is more prevalent in north part of country. in order to determine the distribution of soft ticks, argasidae, and their infection with borrelia species in hamadan province, 53 villages were selected randomly. a total of 4805 ticks were collected directly from human dwellings, poultries, and an...

background: west azerbaijan is considered as a main region for domestic animal breeding. due to importance of herd as a main host and ticks as a vector of relapsing fever and cchf, a comprehensive study was undertaken in the region. methods: outdoor, indoor collection as well as ticks stick to the animals' body were collected and identified. the study was conducted during the whole seasons in 2...

Several drivers have recently fostered the expansion of Angiostrongylus vasorum throughout Europe, where Vector-Borne Pathogens (VBPs) are also spreading. However, level simultaneous risk infection is still unknown in canine populations. This study evaluated exposure to A. and major VBPs dogs Italy. Sera 294 were subjected two ELISAs, detecting circulating antigens antibodies against parasite, ...