There is great interest in how bone marrow derived stem cells make fate decisions. Numerous studies have investigated the role of individual growth factors on mesenchymal stem cell differentiation, leading to protocols for cartilage, bone and adipose tissue. However, these protocols overlook the role of biomechanics on stem cell differentiation. There have been various studies that have applied...

Somatic tissue engraftment was studied in BXSB mice treated with mesenchymal stem cell transplantation. Hosts were conditioned with nonlethal radiation prior to introducing donor cells from major histocompatibility complex-matched green fluorescent protein transgenic mice. Transplant protocols differed for route of injection, ie, intravenous (i.v.) versus intraperitoneal (i.p.), and source of m...

in this center, from 1991 to 2002, 89 chronic myelogenous leukemic (cml) patients, age ranging between 8-48 years with a median age of 29, underwent hematopoietic stem cell transplantation. eighty-eight patients were in the first chronic phase of disease. twenty-three patients received bone marrow transplantation (bmt) and 66 patients received peripheral blood stem cell transplantations (pbsct)...

In this paper, we established a delayed wound healing model on diabetic rat to mimic the pathophysiology of clinical patients who suffered from diabetic foot ulcers. We also evaluated if transplantation of allogeneic bone marrow-derived mesenchymal stem cells could promote the delayed wound healing and investigated the possible underlying biological mechanisms and stem cell behavior involved in...

most studies regarding the marrow-derived equine mesenchymal stem cells (mscs) have mainly focusedon the cell transplantation without considering the capacity of differentiation and in vitro requirements of thecells. these concerns were investigated in the present study. equine mscs were isolated from the sternalmarrow aspirates and expanded through two successive subcultures. passage-2 equine ...

Most studies regarding the marrow-derived equine mesenchymal stem cells (MSCs) have mainly focusedon the cell transplantation without considering the capacity of differentiation and in vitro requirements of thecells. These concerns were investigated in the present study. Equine MSCs were isolated from the sternalmarrow aspirates and expanded through two successive subcultures. Passage-2 equine ...

OBJECTIVE To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center. METHODS A retrospective study was carried out of 21 candidates for cell therapy. After the isolation of bone marrow mononuclear cells by density gradient, mesenchymal stem cells were cultivated and expanded at ...

objective(s) arsenic compounds are potent human carcinogen and produce a variety of stress responses in mammalian cells. recently sodium arsenite has been recommended to be used as anti malignancy drug by american food and drug administration (fda). in this study, we aimed to determine the apoptosis inducing effect of sodium arsenite on rat bone marrow mesenchymal stem cells exposed in vitro. m...

Objectives- To isolate, culture-expand and differentiate mesenchymal stem cells from ovine bone marrow and determine their culture requirements for high expansion rate. Design- Experimental study. Animals- Five Shal sheep. Procedures- In this study, ovine marrow cells were plated and culture expanded through 3 successive subcultures. The resultant cells were then plated at differentiating condi...

Objective: The aim of this work was to explore the therapeutic effect of bone marrow-derived mesenchymal stem cells transplantation in SD rats’ ischemic hind limb. Methods: Ligation of the abdominal aorta beneath the renal artery, the lumbar artery and the iliolumbar artery, was performed to obtain the hindlimb ischemia model in female SD rats. Amplified and purified bone marrow-derived mesench...