Synthesis of long peptides is generally considered as a challenge to peptide chemists, in addition producing significant amounts toxic waste, such DMF. Here we show that using solvent-less methods, ball milling, enabled the production hexapeptide Boc-(Ala-Phe-Gly)2-OBn at gram scale with high overall yield (77 %, 5 linear steps). This longest chain synthesized mill date, which amino acid sequen...
