This study compared the field fertility outcomes in frozen-thawed (FT) sperm from boar ejaculates with different freezability (good, GFE/poor, PFE) while testing the reliability of the post-cervical artificial insemination (post-CAI) in FT sperm. The assay was conducted over eight months with 86 weaned sows being inseminated by post-CAI. Every ejaculate in a total of 26 from 15 Piétrain boars w...

The human and murine genes for MRP9 (multidrug resistance-associated protein 9; ABCC12) yield many alternatively spliced RNAs. Using a panel of monoclonal antibodies, we detected full-length Mrp9 only in testicular germ cells and mouse sperm; we obtained no evidence for the existence of the truncated 100 kDa MRP9 protein reported previously. In contrast with other MRPs, neither murine Mrp9 nor ...

In order to extend the static information of immunolabelling sulphogalactolipids in fixed boar spermatozoa, a fluorescent sulphogalactolipid analogue, galactose(3-sulphate)-beta 1-1'[(N-lissamine rhodaminyl)-12-aminodode-canoyl]-sphingosine, was incorporated into plasma membranes of living spermatozoa and its lateral distribution over the sperm head was studied. The fluorescent lipid was enrich...

Affinity chromatography on Chelating Sepharose Fast Flow Gel-Zn(2+) was used for fractionation of boar seminal plasma proteins. Approximately 30% of total boar seminal plasma proteins showed affinity for zinc ions (ZnBP fraction). Native electrophoresis (PAGE) of ZnBP revealed six protein fractions which separated into 27 bands under denaturing conditions (SDS/PAGE). Two-dimensional electrophor...

KONDRACKI, S., M. IWANINA and A. WYSOKINSKA, 2015. Assessment of the relationship between physical ejaculate parameters and the sexual activity of Landrace boars. Bulg. J. Agric. Sci., 21: 882–888 The study involved 11 Landrace boars, aged 8-9 months and used for insemination purposes. Each boar was assessed four times for its sexual activity: at the beginning of breeding service and after 3, 6...

Without question, the implementation of artificial insemination (AI) has been the greatest achievement in swine reproductive technology during the last decade. Because AI in swine is not a new technology, the underlining technological enhancements of this procedure are most likely responsible for its recent popularity. It is widely accepted that reproductive performance as a result of AI can be...

Current Status of AI Use The USA swine breeding herd has been reported to include 3.9 million sows. Approximately 60% of these sows were determined to have been mated by artificial insemination. Each sow received 6.5 doses of semen annually, for a total of 25 million doses utilized in a single year (1999, Boar Semen Conference). Most of the AI is performed with 3 billion sperm in 80 cc of a 5-d...

The effects of different freezing and thawing rates on the post-thaw motility and membrane integrity of boar spermatozoa, processed as split samples in Maxi-straws or flat PET-plastic packages (FlatPack) were studied. A programmable freezing device was used to obtain freezing rates of either 20, 50 or 80 degrees C/min. Thawing of the samples was performed in a bath of circulating water; for 40s...

The study evaluated the protective effect of seminal plasma (SP) added to freezing extender against cryopreservation injuries to boar spermatozoa. Pooled sperm-rich fractions collected from 9 fertile boars were frozen in 0.5-mL straws after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1-SP4) collected from the sperm-rich fractions (diluted 1:...