An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acet...

The studies to be reported were undertaken with the idea of throwing more light on the proteins of the gastric juice. It has been found that they can be precipitated by tungstic acid at pH 3.5 and that, although heating coagulated a protein, there remained in solution a heat-uncoagulable substance which gave a positive biuret reaction. Interest in the subject was stimulated by the investigation...

The globulin content of the standard solution in the vials is standardized either by a Kjeldahl or a biuret determination. When available Lederle (gamma) globulin diluted 1:200 can be used for this purpose as well. The diluted Lederle standard globulin coagulates on heating and so cannot be used as a sterilized standard. An interesting phenomenon was observed while preparing the sterilized wate...

This study was designed to evaluate the amounts of coagulation factors and to determine whether the protein profile in pre-ovulatory ovarian follicular fluid aspirated from ovaries collected from mares at slaughter are representative of that in follicular fluid collected from live animals. The proteins evaluated included, (i) albumin, ceruloplasmin and fibronectin, (ii) the procoagulant plasma ...

OBJECTIVES To examine if car painters who work with polyurethane paints that contain hexamethylenediisocyanate (HDI) and hexamethylenediisocyanate biuret trimer (HDI-BT) develop acute as well as chronic impairment of lung function. METHODS In this study data were reanalysed from two earlier studies on a group of car painters to see if a decrease in lung function within the week is a marker of...

1. The rate of hydrolysis at different pH values of glycyl glycine, glycyl leucine, glycyl alanine, glycyl asparagine, glycyl aspartic acid and biuret base has been determined. 2. The pH-activity curves obtained in this way differ for the different substrates. 3. The curves can be satisfactorily predicted by the assumption that erepsin is a weak acid or base with a dissociation constant of 10(-...

We evaluated four spectrophotometric methods for suitability in determining total protein in human milk samples. Results were compared with those by an established method for the determination of total nitrogen (micro-Kjeldahl). Values for total protein by all four methods significantly correlated with micro-Kjeldahl. For the biuret assay, Lowry-Peterson assay, Bio-Rad Coomassie Blue assay, and...

Cyanuric acid hydrolases are of industrial importance because of their use in aquatic recreational facilities to remove cyanuric acid, a stabilizer for the chlorine. Degradation of excess cyanuric acid is necessary to maintain chlorine disinfection in the waters. Cyanuric acid hydrolase opens the cyanuric acid ring hydrolytically and subsequent decarboxylation produces carbon dioxide and biuret...