Frozen solutions of biotinylated glucose-6-phosphate dehydrogenase and fluorescently tagged avidin were exposed to high energy ionizing radiation. Parallel experiments with peroxidase coupled to streptavidin and with biotinylated phycoerythrin were also performed. The loss of function of each compound was analyzed according to target theory. Target analysis revealed that the radiation-sensitive...

Vascular proteins expressed at liver metastasis sites could serve as prognostic markers or as targets for pharmacodelivery applications. We employed a proteomic approach to define such proteins in three syngeneic mouse models of liver metastasis. Vascular structures were biotinylated in vivo by a terminal perfusion technique, followed by mass spectrometric analysis of accessible biotinylated pr...

The reciprocal connections between primary motor cortex (M1) and primary somatosensory cortex (S1) are hypothesized to play a role in an animal's ability to update its motor plan in response to changes in the sensory periphery. These interactions provide the sensory cortex with anticipatory knowledge of motor plans. In the mouse neocortex there are representations of the body surface within bot...

Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins regulate biological functions, such protein–protein interactions. PP-InsPs strongly negatively charged and interact with basic surface patches proteins, making their quantitative biochemical analysi...

A caspase-3 controlled condensation was applied to self-assemble biotinylated nanoparticles for capturing FITC-labelled streptavidin and subsequently turning on the fluorescence signal.