Abstract Keroplatus is a genus of fungus gnats family Keroplatidae (Diptera, Bibionomorpha). Larvae some species emit constant blue light from the body. The bioluminescence one least studied all terrestrial insects and very few facts are known to date its biology biochemistry. Here we report high level riboflavin in testaceus larvae, fluorescent compound that might be relative bioluminescent sy...

The presence of two spectral mechanisms, near-ultraviolet and green (lambda(max)=545nm), is strongly suggested by electroretinographic visual spectral sensitivity curves obtained under dark and red chromatic adaptation conditions in the compound eyes of the click beetle Pyrophorus punctatissimus. The bioluminescence emission of the dorsal prothoracic lanterns is deep green (lambda(max)=543nm) a...

In this paper, we report the synthesis of tin oxide (SnO2) nanoparticles by co-precipitation technique. The structural, surface morphology, thermal and optical properties of the SnO2 samples were analysed using X-ray diffraction (XRD), High-resolution transmission electron microscopy (HRTEM), Fourier transformed infrared (FTIR) spectrum, thermo gravimetric analysis (TGA/DT...

We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor-composed of a donor luciferase that is genetically fused to a Venus fluorophore-exhibits pH dependence of its spectral emission in vitro. When expressed in either yeast or mammalian cells...

The Hazard Analysis and Critical Control Points (HACCP) system prevents manages physical, chemical biological risks at places where foods beverages are processed, packaged, distributed consumed. present study (1) assessed the level of microbial contamination food contact surfaces using adenosine triphosphate (ATP)-bioluminescence in Iraq restaurants; (2) investigated surfaces; (3) evaluated eff...

Bioluminescence whereby light is emitted as a result of a chemical reaction offers several advantages over other assay methods, such as fluorescence, including low background and the utilization of reagents with extended shelf life [1]. Also, since no filters or excitation sources are needed, lab-on-chip integration is considerably easier. Of special interest are luciferase-based assay methods ...

nanoparticles of zirconium(iv) tungstate are prepared by chemical co-precipitation method. thecharacterizations have been carried out by tg/dta, dsc, afm, xrd, ftir and uv-vis spectrum. plspectrum shows two emission bands at 350 nm and at 477 nm. the effects of frequency on the dielectricbehavior and ac electrical conductivity have been studied. the dielectric constant is found to be 119.43 at1...

pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and adp to yield atp and pyruvate. geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. given that limited proteolysis experiments can be successfully used to probe conformational features of p...

The first systematic steady-state and time-resolved emission study of firefly oxyluciferin (emitter in firefly bioluminescence) and its analogues in aqueous buffers provided the individual emission spectra of all chemical forms of the emitter and the excited-state equilibrium constants in strongly polar environment with strong hydrogen bonding potential. The results confirmed the earlier hypoth...