Materials and methods: Classical biochemical methods were used to determine plasma malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) levels. Enzyme-linked immunosorbent assay was used to determine plasma insulin levels and reverse transcriptasepolymerase chain reaction was used to determine the levels of ...

Clinical and biochemical evidence of a catecholamine-secreting tumour was documented in a 19-year-old female presenting with malignant hypertension. The tumour was successfully localized to the pelvis by means of plasma noradrenaline assay from multiple sites, after previous attempts at localization had failed. Subsequently, computed tomographic (CT) scan allowed correct pre-operative localizat...

Enterococcus faecium has recently emerged as a serious nosocomial pathogen. The prevalence and severity of enterococcal infections, the mortality rate from such infections, and the antibiotic resistance of enterococci are often species dependent. Since conventional biochemical methods fail to differentiate E. faecium from certain newly described enterococcal species, a PCR-based assay was devel...

The rate of false-positive hepatitis C virus enzyme immunoassay results was determined to be at least 10% among 1,814 reactive serum samples based on (i) negative results in an independent confirmation assay, (ii) negative PCR results, and (iii) no patients developing clinical or biochemical signs of hepatitis during a 1-year follow-up.

A case of haemolytic uraemic syndrome in a three year old boy is described. The clinical course was complicated by hyperglycaemia, and biochemical assay of plasma showed an almost total lack of pancreatic insulin. Extensive necrosis of the exocrine and endocrine pancreas was found at necropsy. The possible pathological physiology of this complication and its prognostic importance are discussed.

mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. in the present study nasal swab samples (n=778) were processed and 51isolates of m. ovipneumoniae were identified through biochemical tests and pcr. difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. similarly the isolate rate among healthy (n=513) and resp...

considerable amounts of different pollutants, especially crude oils, are present in the persian gulf and its northern region, so biomonitoring the effect of these pollutants on its biota is necessary. ethoxyresorufin o-deethylase (erod), gluthathione s-transferase (gst) and catalase (cat) activity were measured from biochemical level in the gills of mudskipper periophthalmus waltoni collected f...

Opa1, also known as Mgm1 in yeast, is a mitochondrial member of the dynamin family. Unlike other dynamin family members, Opa1 has an N-terminal mitochondrial targeting sequence, suggesting that this protein is imported into mitochondria. Here, we describe biochemical techniques, such as mitochondrial isolation, digitonin extraction, a protease protection assay, and carbonate extraction, that we...

In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activ...

Chlorine dioxide is a disinfectant used worldwide. In this article, a new enzymatic method for the determination of chlorine dioxide has been developed. This rapid spectophotometric assay is able to detect from 0.2 to 4 mg/liter of chlorine dioxide. The method is based on the capacity of horseradish peroxidase to decolorize reactive yellow 17 in the presence of chlorine dioxide. The effects of ...