Developing siRNA therapeutics poses technical challenges including appropriate molecular design and testing in suitable pre-clinical models. We previously detailed sequence-selection and modification strategies for siRNA candidates targeting STAT6. Here, we describe methodology that evaluates the suitability of candidate siRNA for respiratory administration. Chemically-modified siRNA exhibited ...

Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...

Pyrolysis of Alcea pallida stems was performed in a fixed-bed tubular reactor with and without catalyst at three different temperatures. The effects of pyrolysis parameters including temperature and catalyst on the product yields were investigated. It was found that higher temperature resulted in lower liquid (bio-oil) and solid (bio-char) yields and higher gas yields. Catalysts had different e...

Recently there is a great demand for extending high throughput life science research to biomanufacturing. However, there is a gap from life science research to bio-manufacturing. Most of existing Bio-workflow tools/systems only provide an isolated solution to help bioscientist to orchestrate bio-R&D operations such as bio-database query, bio-computation and analysis etc for specific small bio-p...

Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine vir...

this study was conducted to compare the bio-efficacy of herbal methionine (h-met) relative to dl-methionine (dl-met) on 160 “ross 308” broiler chickens. dl-met and h-met were added to the basal diet in eight experimental treatments with three and four concentrations respectively in starter, grower and finisher period. blood parameters which were measured at 24 and 42 days of age consisted of: s...

this experiment was laid out in order to evaluate the effects  of different biofertilizers on yield components of barley. the experiment was a factorial design with three replications. treatments were three nitrogen biofrtilizers (nitroksin, nitrokara and supernitroplass) and three phosphate  biofrtilizers (phosphate barvar2, biozarr and superplass) with control for them and yield components we...

this experiment was lay out in order to evaluate the effects of different biofertilizers on seed yield, protein and oil of rapeseed in iran. the experiment was a factorial design with three replications. treatments were four nitrogen biofrtilizers (control (n1), nitroksin (n2), azotobacter (n3) and supernitroplat (n4)) and three phosphate biofrtilizers ( control(p1), biosfer phosphate (p2) and ...

normal 0 false false false en-us x-none ar-sa background: dry weight (dw) is an important concept related to patients undergoing hemodialysis. conventional method seems to be time consuming and operator dependent. bio impedance analysis (bia) is a new and simple method reported to be an accurate way for estimating dw. in this study, we aimed to compare the conventional estimation of dw with mea...

the objective of this research is to investigate the effect of four additives including iron slag (is), works debris (wd), fly ash (fa), and lime kiln dust (lkd) on the biochemical properties of biosolids produced at mangere wastewater treatment plant (mwwtp), auckland, new zealand. all these additives are homogenously mixed in the laboratory with biosolids at various percentages with and witho...