apotransferrin (apo tf)  in 0.1 m n-(2hydroxyethyl)piperazine-n2-ethanesulfanic acid at 25 ˚c  and ph 7.4 has been titrated with acidic solution of tb3+. the binding of tb3+ at  the two specific metal-binding sites of transferrin was followed from the changes in the difference uv spectra at 245 nm. the molar absorptivity per binding site for tb3+-tf is 22,500 ± 1000 m-1cm-1. to determine the tb...

Structural models with no solution are incoherent, and those multiple solutions incomplete. We show that occasionally binding constraints not generically coherent. Coherency requires restrictions on the parameters or support of distribution shocks. In presence shocks, cannot be independent from each other, so assumption orthogonality structural shocks is incompatible coherency. Models whose coh...

MC-TAG derivations using delayed locality (i.e. non set-local composition) have been used to model long distance binding in natural language. In this paper, I explore the possibility within Synchronous TAG that tree sets composing via delayed locality may have many possible syntactic derivations for a single semantic form. Using the Mandarin (Chinese) anaphor ziji as a test case, I show that th...

approximately all sequenced archaeal and half of eubacterial genomes have some sort of adaptive immune system, which enables them to target and cleave invading foreign genetic elements by an rnai-like pathway. crispr–cas (clustered regularly interspaced short palindromic repeats–crispr-associated proteins) systems consist of the crispr loci with multiple copies of a short repeat sequence separa...

Approximately all sequenced archaeal and half of eubacterial genomes have some sort of adaptive immune system, which enables them to target and cleave invading foreign genetic elements by an RNAi-like pathway. CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems consist of the CRISPR loci with multiple copies of a short repeat sequence separa...

The sialic acid-binding lectin sialoadhesin (Sn) is a macrophage-restricted receptor for porcine reproductive and respiratory syndrome virus (PRRSV). To investigate the importance of pSn sialic acid-binding activity for PRRSV infection, an R(116)-to-E mutation was introduced in the predicted sialic acid-binding domain of pSn, resulting in a mutant, pSn(RE), that could not bind sialic acids. PSn...

Abstract The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest ~ 300 amino acid receptor binding domain (RBD) within S1 subunit spike protein because its key interaction with human angiotensin converting enzyme 2...

We present a systematic reconstruction of a compilation method for an extension to logic programming that permits procedure de nitions to be given a scope. At a logical level, this possibility is realized by permitting implications to be embedded in goals. Program clauses that appear in the antecedents of such implications may contain variables that are bound by external quanti ers, leading to ...

The local variational method is a technique to approximate an intractable posterior distribution in Bayesian learning. This article formulates a general framework for local variational approximation and shows that its objective function is decomposable into the sum of the Kullback information and the expected Bregman divergence from the approximating posterior distribution to the Bayesian poste...