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Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...
Heidegger’s Art thinking is the result of his later thought. But the death-thinking is attendant with “Being thinking” in the whole of his thought. In fundamental ontology, “Being” is understood through “Dasein”. The problem of Dasein historicity and temporality led heidegger to contemplate about death. After “turning,” death thinking, it was followed through the “Event (Ereignis) thinking”. In...
introduction acetic acid bacteria are large group of obligate aerobic gram negative bacteria with the ability to oxidize ethanol to acetic acid (1). they are widely distributed in natural habitats and classified in family acetobacteraceae. members of this family are useful in industrial production of vinegar(2). acetic acid bacteria (aab) can use substrates as glucose, ethanol, lactate or glyc...
Muhammad Iqbāl of Lahore(1877-1938) is undoubtedly the greatest Muslim poet-thinker of the twentieth century. Iqbāls philosophical writings and poetical works had a noteable impression on the religio-cultural and social revival of the East particularly subcontinent Muslim. The central theme of Iqbāls philosophical thought throughout his works, prose and poetry, especially in Asrar-i-Khudi ...
when kafka started to write the metamorphosis as an allegory in 1912, he had a concealed purpose and aim in his mind. he has described his own idea and somehow himself in metamorphosis. in other words, his attitude in metamorphosis is the reflection of himself. gregor samsa, the main character in the story, is a hardworking person who loves his family. he takes no notice of enjoyment in his lif...
One of the important issues in medical ethics is the way of treating and making decision on a patient’s treatment, especially if a physician encounters with some limitations to make a decision. In new medical ethics, the “Autonomy Principle” (respect for patient’s autonomy and individual autonomy) is used to solve the problem; that is, physicians decide on whether to treat a patient or not afte...
methodology non-native participants in this study were sixty ba students majoring in english literature and teaching english as a foreign language at khayyam university of mashhad. they were senior students, between 21 to 24 years old, who had studied english for at least three and a half years and had passed several courses including grammar, reading, conversation, and writing. this was assum...
in this project, 4,4?-methylenebis(3-chloro-2,6-diethyl trimellitimidobenzene) as a diacid, was prepared by the reaction of trimellitic anhydride (tma) with 4,4?-methylenebis(3-chloro-2,6-diethylaniline) (mcdea). the novel poly(amide-imide) (pai) was synthesized from the reaction of 4,4?-methylenebis(3-chloro-2,6-diethyl trimellitimidobenzene) as a diacid with mcdea under green condition using ...
human history has been witness to the emergence of great men and women who illustrated the sufferings and wishes of mankind. they have bravely put in hard effort to resist long-standing and reactionary traditions of their own society and to fight oppressors, and at the same time, the comfort-seeking opportunists have endeavored to derail their efforts and to project a tarnished image of these c...
Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the ...
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