نتایج جستجو برای: bc1
تعداد نتایج: 1088 فیلتر نتایج به سال:
The mode of membrane attachment of the Rieske iron-sulfur protein from cytochrome bc1 complex of Rhodospirillum rubrum has been studied using biochemical approaches. In contrast to cytochrome c1 the bacterial Rieske protein was extracted from chromatophores using chaotropic agents (NaSCN, urea, guanidine), an alkaline pH and relatively low concentration of Triton X-100. The results presented he...
RNA interference has become a powerful tool for the specific inhibition of genes in mammalian cells. Previously, Alu-linked hairpins were demonstrated to transiently mediate RNA interference without associated toxicity in contrast to hairpins expressed from upstream RNA pol III promoters. To expand upon those results, we now demonstrate that hairpins attached to the ID portion of the rat neuron...
The objectives of this study were to evaluate the tolerance to low temperatures and tuber soft rot in hybrids between Solanum com! mersonii and Solanum tuberosum[ The experimental materials consisted of F0 triploid\ BC0 pentaploidÐnear pentaploid and BC1 tetraploidÐ near tetraploid hybrids[ The F0 triploids had a freezing tolerance and acclimatization capacity closest to S[ commersonii[ This in...
A BioFocus DPI SoftFocus library of ∼35 000 compounds was screened against Mycobacterium tuberculosis (Mtb) in order to identify novel hits with antitubercular activity. The hits were evaluated in biology triage assays to exclude compounds suggested to function via frequently encountered promiscuous mechanisms of action including inhibition of the QcrB subunit of the cytochrome bc1 complex, dis...
The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX- strains of Rhodobacter sphaeroides. We show that this single polypeptide profoundly affects membrane morphology, leading to highly elongated cells containing extended tubular membranes. Purified tubular membranes contain heli...
The individual components of the photosynthetic unit (PSU), the light-harvesting complexes (LH2 and LH1) and the reaction center (RC), are structurally and functionally known in great detail. An important current challenge is the study of their assembly within native membranes. Here, we present AFM topographs at 12 A resolution of native membranes containing all constituents of the PSU from Rho...
In the cytochrome bc(1) complex, the swivel motion of the iron-sulfur protein (ISP) between two redox sites constitutes a key component of the mechanism that achieves the separation of the two electrons in a substrate molecule at the quinol oxidation (Q(o)) site. The question remaining is how the motion of ISP is controlled so that only one electron enters the thermodynamically favorable chain ...
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