Vaccination is one of the most effective strategies to control epidemics. With deepening people’s awareness vaccination, there a high demand for vaccination. Hence, flexible, rapid, and cost-effective vaccine platform urgently needed. The baculovirus expression vector system (BEVS) has emerged as promising technology production due its safety, rapid production, flexible product design, scalabil...

Innate immunity is essential for insects to survive infectious pathogens. In baculovirus-infected lepidopteran cells, apoptosis and global protein synthesis shutdown are major mechanisms of intracellular innate immunity that inhibit viral replication. In contrast, baculoviruses have evolved diverse genes and mechanisms to counter the antiviral immunity activated in infected cells. In this revie...

CRYSTAL STRUCTURE OF BACULOVIRUS RNA TRIPHOSPHATASE (BVP) COMPLEXED WITH PHOSPHATE* Anita Changela, Alexandra Martins, Stewart Shuman, and Alfonso Mondragón From the Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208 and Molecular Biology Program, SloanKettering Institute, New York, New York 10021 Running Title: Cry...

The 1035 base-pair (bp) open reading frame 59 (ORF59) of channel catfish virus (CCV) codes for an abundant hydrophobic membrane glycoprotein considered to be the major envelope glycoprotein of the virus. The ORF59 gene of CCV was cloned into the baculovirus genome by site-specific transposition and expressed in Spodoptera frugiperda cells (Sf9 cells). Expressed protein could be recognized by po...

Baculoviruses are one of the largest viruses that replicate in the nucleus of their host cells. During infection, the rod-shape, 250-nm long nucleocapsid delivers its genome into the nucleus. Electron microscopy evidence suggests that baculoviruses, specifically the Alphabaculoviruses (nucleopolyhedroviruses) and the Betabaculoviruses (granuloviruses), have evolved two very distinct modes for d...

BACKGROUND Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...