To examine the effect of crop growth enhancer bacteria on quantitative and qualitative yield of safflower, an experiment in randomized complete block design was conducted in eight levels with four replications. Bacterial factor consisted of control (absence of bacteria), AzotobacterChroococcum,AzospirillumBrazilians, Pseudomonas Fluorescens, AzotobacterChroococcum+Azospirillum Brazilians, Azoto...

Cys2His2 zinc-fingers (C2H2 ZFs) mediate a wide variety of protein-DNA and protein-protein interactions. DNA-binding C2H2 ZFs can be shuffled to yield artificial proteins with different DNA binding specificities. Here we demonstrate that shuffling of C2H2 ZFs from transcription factor dimerization zinc-finger (DZF) domains can also yield two-finger DZFs with novel protein-protein interaction sp...

Introduction: Gastrointestinal infection is a major cause of morbidity worldwide. Culture and microscopy are time consuming have low diagnostic yield. New rapid molecular methods such as multiplex PCR recently been introduced for etiological diagnosis. The purpose this study was to compare the yield FilmArray gastrointestinal panel with that standard culture diagnosis infections. Materials meth...

Sugar beet tailings were anaerobically digested at non-agitated and agitated conditions in identical thermophilic batch reactors. The average methane yield in the agitated digester was only 74% of that in the non-agitated digester. Ninety percent of the ultimate methane yield was produced in approximately 5 days in the non-agitated digester whereas it took 12 days in agitated digester. Even upo...

That bacterial derivatives possess anaphylactogenic properties was demonstrated by the pioneers in the study of anaphylaxis. But from the numerous subsequent studies in bacterial hypersensitiveness the fact has emerged that these antigens are not highly endowed with anaphylactogenic properties. Compared to such substances as egg albumin and animal serums bacterial derivatives yield positive res...

The global rise of anti-microbial resistance, combined with the rapid rate of microbial evolution, and the slower development of novel antibiotics, underscores the urgent need for innovative therapeutics. We are facing a post-antibiotic era with a decreased armamentarium to combat infectious diseases. Development of novel drugs will rely on basic research aimed to increase our understanding of ...

Ralstonia solanacearum is one of the most devastating bacterial plant pathogens due to its large host range, worldwide geographic distribution and persistence in fields. This soilborne pathogen is the causal agent of bacterial wilt and it can infect major agricultural crops thereby reducing significantly their yield. To favor infection, the bacterium delivers, through the type three secretion s...

Trigger factor and DnaK protect nascent protein chains from misfolding and aggregation in the E. coli cytosol, but how these chaperones affect the mechanism of de novo protein folding is not yet understood. Upon expression under chaperone-depleted conditions, multidomain proteins such as bacterial beta-galactosidase (beta-gal) and eukaryotic luciferase fold by a rapid but inefficient default pa...

Recent molecular studies on endophytic bacterial diversity have revealed a large richness of species. Endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. Some endophytes are seedborne, but others have mechanisms to colonize the plants that are being studied. Bacterial mutants unable t...

Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure. However, it has been shown in many cases that a synthetic fragment of appropriate structure conj...