objective(s): occludin is essential for proper assembly of tight junctions (tjs) which regulate paracellular endothelial permeability. omega-3 polyunsaturated fatty acids (ω-3 pufa) protect endothelial barrier function against injury. materials and methods: we examined anti-inflammatory effect of ω-3 pufa intake (30 mg/kg/day for 10 days) on expression and location of occludin in the aorta of a...

The influence of various carbon sources as bioflocs on relative immunological gene expression, haematology, growth, and microbial community in Clarias gariepinus juvenile culture is investigated this study. groups (four) were created by daily supplementation with four (cassava peel, tapioca, wheat offal, brewery waste) a carbon-nitrogen ratio 20 the control without addition. (8.16 ± 0.2 g) was ...

Bacterial response regulators (RR) that function as transcription factors in two component signaling pathways are crucial for ensuring tight regulation and coordinated expression of the genome. Currently, consensus DNA binding sites in the promoter for very few bacterial RRs have been identified. A systematic method to characterize these DNA binding sites for RRs would enable prediction of spec...

The impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. In this study, bacterial system has been developed for expression and purification of properly folded HA1 antigen as a rapid response to emerging pandemic strains. Here, a recombinant H5N1 (A/Indone...

Type III secretion systems (T3SSs) are crucial for bacterial infections because they deliver effector proteins into host cells. The Escherichia coli type III secretion system 2 (ETT2) is present in the majority of E. coli strains, and although it is degenerate, ETT2 regulates bacterial virulence. An ATPase is essential for T3SS secretion, but the function of the ETT2 ATPase has not been demonst...

Background: Expression of virus coat protein (CP) in Escherichia coli often leads to production of partially folded aggregated proteins which are called inclusion bodies. Grapevine fanleaf virus (GFLV) is one of the most serious and widespread grapevine virus diseases around the world and in Iran.Objective: The main objective of this study was to find a s...

The bacterial transcriptome is a dynamic entity that reflects the organism's immediate, ongoing and genome-wide response to its environment. Microarray expression profiling provides a comprehensive portrait of the transcriptional world enabling us to view the organism as a 'system' that is more than the sum of its parts. The vigilance of microorganisms to environmental change, the alacrity of t...

While very much impressed by the scope of the chimical reaction models handled by Professor Wilkinson, we will (presumably predictably!) focus on the simulation aspects of his paper. First, the solution proposed by the author to overcome the difficulties of handling the complex likelihood π(x|θ) reminds us of the auxiliary completion of Møller et al. (2006), who created (as well) an auxiliary d...