Title of Thesis: DEVELOPING TOOLS FOR INVESTIGATING CHEMOTAXIS SIGNAL CLUSTERS IN BACILLUS SUBTILIS James A. Rogers, Jr., Master of Science, 2012 Thesis directed by: Associate Professor Richard C. Stewart Department of Cell Biology and Molecular Genetics Many bacteria make use of a set of dedicated chemoreceptor proteins to control a His-Asp signaling system; this control converts environmental...

By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of producti...

Bacillus spp. are widely used as feed additives and probiotics. However, there is limited information on their resistance to various antibiotics, and there is a growing concern over the transfer of antibiotic resistance genes. The MIC for 8 antibiotics was determined for 85 Bacillus species strains, Bacillus subtilis subsp. subtilis (n = 29), Bacillus licheniformis (n = 38), and Bacillus sonore...

Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identified the RLP of B. subtilis as the 2,3-diketo-5-methylthiopentyl-1-phosphate en...

The ftsL gene is required for the initiation of cell division in a broad range of bacteria. Bacillus subtilis ftsL encodes a 13-kDa protein with a membrane-spanning domain near its N terminus. The external C-terminal domain has features of an alpha-helical leucine zipper, which is likely to be involved in the heterodimerization with another division protein, DivIC. To determine what residues ar...

In this study; a total of seven bacterial strains (Bacillus cereus FD-63, Bacillus sphaericus FD-49, subtilis EK-7, thuringiensis subsp. kurstakii FDP-41, Brevibacillus brevis CP-1, Pseudomonas chlororaphis Nem-28 and fluorescens KŞN-1) one fungus isolate (Beauveria bassiana ET-10) were tested for determining biological control activity against on mature (Leptinotarsa decemlineata Say) 1., 2. 3...

Part 1 issued November 1971 PAGE Effects of Organochlorine Insecticides on Bacterial Growth, Respiration and Viability. By P. W. TRUDGILL, R. WIDDUS and J. S. REES The Effects of Technical Chlordane on Energy Metabolism of Bacillus subtilis. By R. WIDDUS, P. W. TRUDGILL and M. J. MALISZEWSJSI The Effects of Technical Chlordane on Growth and Energy Metabolism of Streptococcus faecalis and Mycoba...

Herein we outline the antibacterial activity of amino acid containing thiazolidinediones and rhodanines against Gram-positive bacteria Staphylococcus aureus ATCC 31890, Staphylococcus epidermidis and Bacillus subtilis ATCC 6633. The rhodanine derivatives were generally more active than the analogous thiazolidinediones. Compounds of series 5 showed some selectivity for Bacillus subtilis ATCC 663...

1. In relatively low concentrations of NaCl, KCl, and CaCl(2) the rate of respiration of Bacillus subtilis remains fairly constant for a period of several hours, while in the higher concentrations, there is a gradual decrease in the rate. 2. NaCl and KCl increase the rate of respiration of Bacillus subtilis somewhat at concentrations of 0.15 M and 0.2 M respectively; in sufficiently high concen...

In this study, we have shown the microbial alkaline protease production by using isolated Bacillus subtilis. Bacillus subtilis which produces an extracellular alkaline protease was isolated from meet surface purchased from local Egyptian markets. Maximum enzyme activity was achieved when the bacterium was grown on corn steep liquor (2.0 %, w/v) instead of soybean meal (2 %) followed by casein h...