AmyL, an extracellular alpha-amylase from Bacillus licheniformis, is resistant to extracellular proteases secreted by Bacillus subtilis during growth. Nevertheless, when AmyL is produced and secreted by B. subtilis, it is subject to considerable cell-associated proteolysis. Cell-wall-bound proteins CWBP52 and CWBP23 are the processed products of the B. subtilis wprA gene. Although no activity h...

The complete nucleotide sequence of ermA, the prototype macrolide-lincosamide-streptogramin B resistance gene from Staphylococcus aureus, has been determined. The sequence predicts a 243-amino-acid protein that is homologous to those specified by ermC, ermAM, and ermD, resistance determinants from Staphylococcus aureus, Streptococcus sanguis, and Bacillus licheniformis, respectively. The ermA t...

21 2,3-Butanediol (2,3-BD) is an important starting material for the manufacture of bulk 22 chemicals. For efficient and large-scale production of 2,3-BD through fermentation, 23 low-cost substrates are required. One such substrate, inulin, is a polydisperse fructan 24 found in a wide variety of plants. In this study, a levanase with high inulinase activity 25 and high pH and temperature stabil...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

Pungency of hot pepper has limited its usage even though it shows various health beneficial effects. This study was conducted to develop the novel yoghurt containing hot pepper with diminishing pungency and aimed to examine the quality characteristics of yoghurt prepared with fermented red pepper. Hot pepper was first fermented with Bacillus licheniformis SK1230 to reduce the pungency of capsai...

Composite manufacturing appears to be one of the most suitable recycling options for chromated copper arsenate (CCA)-treated waste wood. However, in many applications it would be beneficial to remove much or all of the CCA from the wood prior to composite production. This paper evaluates the effect of remediation processes, namely oxalic acid (OA) extraction and Bacillus licheniformis fermentat...

Remediation processes for recovery and reuse of chromated-copper-arsenate- (CCA) treated wood are not gaining wide acceptance because they are more expensive than landfill disposal. One reason is the high cost of the nutrient medium used to culture the metal-tolerant bacterium, Bacillus licheniformis, which removes 70-100% of the copper, chromium, and arsenic from CCA-treated southern yellow pi...

Cell extracts of Bacillus licheniformis were found to contain nicotinamide adenine dinucleotide (NAD)-dependent l-alanine dehydrogenase (ADH) (l-alanine: NAD oxidoreductase, EC 1.4.1.1). High specific activities (3.5 to 6.0 IU/mg of protein) were found in extracts of cells throughout growth cycles only when l-alanine served as the primary source of carbon or carbon and nitrogen. Specific activi...

AIMS Isolation of bacterial antagonist for use in the biological control of phytopathogenic fungi like rice blast fungus, Magnaporthe grisea, and to further purify and characterize the antifungal molecule produced by the antagonist. METHODS AND RESULTS Bacterial antagonist exhibiting highest antifungal activity against the rice blast fungus M. grisea was isolated from soil and identified as B...

Poly-γ-glutamic acid (γ-PGA), one type of amino acid polymers, consists of D-and L-glutamic acids linked with amide bonding between α-amino and γ-carboxylic acids. Naturally, γ-PGA could be biodegradable polymer and water-solubleapplied in various industries such as food, cosmetics, and pharmaceutical industries. Variation of initial L-glutamic acid concentration affecting on the production of ...